Phosphatase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_04475T0
SpeciesCryptococcus neoformans var. grubii H99
Gene Annotationhypothetical protein
Gene SymbolHPP3
Phosphatase NameN/A
Phosphatase ClassHistidine phosphatase
KEGG Linkout KEGG::CNI00820 
Genome Browser JBrowse link goes here.
Protein Domain / KEGG Annotation N/A
Amino Acid SequenceMAQKTRPSEKRIHLTRHAQAEHNVADDYTIADAPLTTLGQEQSRQLNEATKNGVQKTAELLVTSPLRRPLETMLLGYPELKSRLEKSGKHAILLDILQEVGPYPCDTPTHPISALKASNGGIFSSLDFSALSPDYASKEGIFAPANGVARAKLVRKWLRERPEREIVVVAHGDILRYIADGQQSSRPWNNAENKVFTFRSDDDSDDSMVEADYNVEARGATNEPTSSEIKAGKF
Expression Data
NotationConditionNormalized Raw Expression
AYPD 30°C, concentration: 2x 10^8 cells/ml5805
BYPD 30°C, concentration: 5x10^7 cells/ml8188
CYPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml8054
DYPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml9840
EYPD 37°C, concentration: 5x10^7 cells/ml7696
FYP+galactose 30°C, concentration: 2x10^8 cells/ml4187
Gene Disruption Strategy
Mutant construction design Mutant construction design Mutant construction design Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_04475 5' flanking region primer 1TGTCCTTGATTCTCTTCGTC
L2CNAG_04475 5' flanking region primer 2TCACTGGCCGTCGTTTTACTCCTCCCTCTGCTATCACTG
R1CNAG_04475 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGTGTTTCGGGTATTGGGCTC
R2CNAG_04475 3' flanking region primer 2CTACATTCCTCCTGTTCACG
SOCNAG_04475 diagnostic screening primer, pairing with B79CACTGGATAGATGATACCGAAC
POCNAG_04475 Southern blot probe primerTGAGAATGCGGGAACTTAC
STMNAT#211 STM primerGCGGTCGCTTTATAGCGATT
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
* Red marked sequences indicate the extended M13 forward and reversed sequences to overlap PCR with pNAT plasmid.
Phenotype Assays
Growth
Growth phenotype
Mating Data
Mating phenotype
Virulence Factor Production
Capsule
Capsule phenotype
Urease
Urease phenotype
Melanin
Melanin phenotype
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype
Oxidative Stress
Oxidative stress phenotype
Heavy Metal Stress
Heavy metal stress phenotype
Cell Wall Stress
Cell wall stress phenotype
ER Stress
ER stress phenotype
Genotoxic Stress
Genotoxic stress phenotype
Antifungal Drug Susceptibility
Antifungal drug susceptibility
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data