Phosphatase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_05529T0
SpeciesCryptococcus neoformans var. grubii H99
Gene Annotationhypothetical protein
Gene SymbolHAD5
Phosphatase NameN/A
Phosphatase ClassHaloacid dehalogenase
KEGG Linkout KEGG::CNH01800 
Genome Browser JBrowse link goes here.
Protein Domain / KEGG Annotation N/A
Amino Acid SequenceMSNPHLKALAITDLKPFFSLSTISHAINMSFRSSHHDGFSGQELEKQPRHTRSGKEWRRYSPSWPVDFSSPPPDYRSYSTSPVRTSRLYHSRSSPSETTRVPWRPPPPPPVSFPPPHYVDLARPSTKKNKALVKKTITPKLLVLDLNGALVWRNRSYRAHMSNPRPYLSCFLEYLFLPETPSDNENKPSDTRSVRPWEVFVWSSAQPHNVRAMVEGCFGSRWIEGIWEQASEDGKKGTQNGEGRLLGVWARDKMGLKGFEYSRKVQTTKDLRKVLSHLIETKKLPDPTTPYSEKTIVLLDDSPLKAIFQPWNQIVIPEFDKALCRSSRLAAGLKSDHYDDYDDGDASTSQIGQGPEMDKILLAVIGILDQLRHVSNVPLWVRAGGLTFDLDETNLHPPTHESLPSHVDYQHWFNDQEVYAKWVAKGEEALERKGIEVRHGLDLLPSPPSRETQGGVFHAAPIAPLRSPSLDSREEREESYTRYSPSRPASCLSPSSSDDHLNLELSEEEVECTKSESAFARRKREERAREKSARKVSKGQQQFAAYAKPKVDEEAINGKIQPSHMADLLTSGNEAIPSESESDGKALDENDIESIAARNKAKQARRKQSRAERKVKTNGKERELEELKRERKEERGSGEAPKKRTLHPRRRRPVKQ
Expression Data
NotationConditionNormalized Raw Expression
AYPD 30°C, concentration: 2x 10^8 cells/ml1324
BYPD 30°C, concentration: 5x10^7 cells/ml1258
CYPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml1172
DYPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml1476
EYPD 37°C, concentration: 5x10^7 cells/ml1794
FYP+galactose 30°C, concentration: 2x10^8 cells/ml1530
Gene Disruption Strategy
Mutant construction design Mutant construction design Mutant construction design Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_05529 5' flanking region primer 1CAAGAGGTCCAAATAGCCTG
L2CNAG_05529 5' flanking region primer 2TCACTGGCCGTCGTTTTACCATTCTTTCCCAGAGCGAG
R1CNAG_05529 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGGAGGCACCAAAAAAGAGAAC
R2CNAG_05529 3' flanking region primer 2CGGTCTTATTACTCTTCGTTCG
SOCNAG_05529 diagnostic screening primer, pairing with B79TCAGCATTGAGAATCCTTCG
POCNAG_05529 Southern blot probe primerTCCATCGTGAACCAAAGC
STMNAT#205 STM primerTATCCCCCTCTCCGCTCTCTAGCA
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
* Red marked sequences indicate the extended M13 forward and reversed sequences to overlap PCR with pNAT plasmid.
Phenotype Assays
Growth
Growth phenotype
Mating Data
Mating phenotype
Virulence Factor Production
Capsule
Capsule phenotype
Urease
Urease phenotype
Melanin
Melanin phenotype
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype
Oxidative Stress
Oxidative stress phenotype
Heavy Metal Stress
Heavy metal stress phenotype
Cell Wall Stress
Cell wall stress phenotype
ER Stress
ER stress phenotype
Genotoxic Stress
Genotoxic stress phenotype
Antifungal Drug Susceptibility
Antifungal drug susceptibility
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data