Phosphatase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_05692T0
SpeciesCryptococcus neoformans var. grubii H99
Gene Annotationsphingosine-1-phosphate phosphatase
Gene SymbolLCB3
Phosphatase NameN/A
Phosphatase ClassLipid phosphatase
KEGG Linkout KEGG::CNF02820 
Genome Browser JBrowse link goes here.
Protein Domain / KEGG Annotation
KEGG Result
KO AccessionE-valueScore
K234512.2e-156519.9
Pfam Result
Pfam AccessionE-valueScore
PF01569 Pfam 1.3e-19 69.1
Amino Acid SequenceMRSSPVIEISPPSSGTAIFQHNPKGDATLRREWEPGCQPDELYDAFLPAWRASLRGLLVRRLRNESTQMADWQKRVRSEARDRYFYWTAVFGTHTFFMMFLPILFFFGHPLEGRGLLHVVGLGIYISSFAKDLVCTPRPYSPPVIRLSMSTHHHEYGFPSSHSTNSVSIALYLGQWMFKLQDRLGWPTVLFSWLMLAVYMTSVIGGRVYTGMHSIADIVGGSIMGVACWLFWIAVGDRNETWVNSGSWTVPAIIAPLGFILIRCHPQPFEACPCFEDAIAVLAVMLGSTLGQWFTVAIWPSIKVQNPTAFYSYNLATIILSIMFRLVFGLGTLFAWRLLAKYTLHQALPPVFRSCSLLLHTSLPARRFYISTAEHNTESSQLALRAIPSLLDLQTGENTETHSAPSCSSPLLTSHALNSRNAIPTSPSSKPGEGLTMRKKSPRENGTERKTATPGEEQAPKSVSALTYDVEVATKVGVYSGIGFIATVVVPYWFELIENTILG
Expression Data
NotationConditionNormalized Raw Expression
AYPD 30°C, concentration: 2x 10^8 cells/ml3808
BYPD 30°C, concentration: 5x10^7 cells/ml3143
CYPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml3429
DYPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml3229
EYPD 37°C, concentration: 5x10^7 cells/ml3053
FYP+galactose 30°C, concentration: 2x10^8 cells/ml3228
Gene Disruption Strategy
Mutant construction design Mutant construction design Mutant construction design Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_05692 5' flanking region primer 1TCTACTACCTTGTCCGACG
L2CNAG_05692 5' flanking region primer 2TCACTGGCCGTCGTTTTACTCTCCTTTGGGATTGTGTTG
R1CNAG_05692 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGCGTCGTTGTTCCCTATTGG
R2CNAG_05692 3' flanking region primer 2TGTCCAACTCCATTTCCCG
SOCNAG_05692 diagnostic screening primer, pairing with B79GTGAAATGAAGGCAGTGAGTTG
POCNAG_05692 Southern blot probe primerTTTGGCACAATGATGAGGG
STMNAT#219 STM primerCCCTAAAACCCTACAGCAAT
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
* Red marked sequences indicate the extended M13 forward and reversed sequences to overlap PCR with pNAT plasmid.
Phenotype Assays
Growth
Growth phenotype
Mating Data
Mating phenotype
Virulence Factor Production
Capsule
Capsule phenotype
Urease
Urease phenotype
Melanin
Melanin phenotype
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype
Oxidative Stress
Oxidative stress phenotype
Heavy Metal Stress
Heavy metal stress phenotype
Cell Wall Stress
Cell wall stress phenotype
ER Stress
ER stress phenotype
Genotoxic Stress
Genotoxic stress phenotype
Antifungal Drug Susceptibility
Antifungal drug susceptibility
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data