Phosphatase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_06064T0
SpeciesCryptococcus neoformans var. grubii H99
Gene Annotationhypothetical protein
Gene SymbolPTP1
Phosphatase NameN/A
Phosphatase ClassProtein tyrosine phosphatase
KEGG Linkout KEGG::CNM00730 
Genome Browser JBrowse link goes here.
Protein Domain / KEGG Annotation
KEGG Result
KO AccessionE-valueScore
K180323.4e-36121
Pfam Result
Pfam AccessionE-valueScore
PF00102 Pfam 2.8e-35 120.2
Amino Acid SequenceMVLICSPHHSLRPEMASIANIERAYRVLADREVTRLEYSLLHKPSTEQAFYSVHNSLKHRRNNRYSNVLAYDRTAVTVDGEYLNANVVTDGKGGRWIAAQAPPPQTFDTFFKALYSGAATGRPPNNVIVVQLTGWEESGIPKADPYMEHKMPDMGVHLSRREIIDRISSTLTEVALQGDRRVNLFHYQFDAWPDHGVPTGTAVQALRELVKEVEEKRRELGCEVWVHCSAGVGRTGTFIALSSLLSPDAPNVSSPIGPLPQEIAGDRIAKTVDTIRESRGMLVQTPGQFELLYRMQ
Expression Data
NotationConditionNormalized Raw Expression
AYPD 30°C, concentration: 2x 10^8 cells/ml3832
BYPD 30°C, concentration: 5x10^7 cells/ml6174
CYPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml7043
DYPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml3445
EYPD 37°C, concentration: 5x10^7 cells/ml3370
FYP+galactose 30°C, concentration: 2x10^8 cells/ml3435
Gene Disruption Strategy
Mutant construction design Mutant construction design Mutant construction design Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_06064 5' flanking region primer 1TTTGGAGGTTCAGGAGTGGC
L2CNAG_06064 5' flanking region primer 2TCACTGGCCGTCGTTTTACTTGTGAAGCAGCGAATACTC
R1CNAG_06064 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGTGCGTTCCTGAAAGTGTCCC
R2CNAG_06064 3' flanking region primer 2GTGATGATTTGCTCGTCG
SOCNAG_06064 diagnostic screening primer, pairing with B79TTGACAGGTGACTGGAAGG
POCNAG_06064 Southern blot probe primerTCCATCTGTTACCACATTGG
STMNAT#125 STM primerCGCTACAGCCAGCGCGCGCAAGCG
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
* Red marked sequences indicate the extended M13 forward and reversed sequences to overlap PCR with pNAT plasmid.
Phenotype Assays
Growth
Growth phenotype
Mating Data
Mating phenotype
Virulence Factor Production
Capsule
Capsule phenotype
Urease
Urease phenotype
Melanin
Melanin phenotype
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype
Oxidative Stress
Oxidative stress phenotype
Heavy Metal Stress
Heavy metal stress phenotype
Cell Wall Stress
Cell wall stress phenotype
ER Stress
ER stress phenotype
Genotoxic Stress
Genotoxic stress phenotype
Antifungal Drug Susceptibility
Antifungal drug susceptibility
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data