Phosphatase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_00146T0
SpeciesCryptococcus neoformans var. grubii H99
Gene Annotation5'/3'-nucleotidase SurE
Gene SymbolPBY101
Phosphatase NameN/A
Phosphatase ClassExopolyphosphatase
KEGG Linkout KEGG::CNA01340 
Genome Browser JBrowse link goes here.
Protein Domain / KEGG Annotation
KEGG Result
KO AccessionE-valueScore
K037875.4e-37125.7
Pfam Result
Pfam AccessionE-valueScore
PF01975 Pfam 2.8e-35 118.9
Amino Acid SequenceMPQLKTYTDKPVVLLTNDDGPPCASSPNIYAFCKLLQSRLGWDVRVVIPDSQKSWVGKSYAISDIVTANYFYPLEPDGLKGEITQTRRPLKEGESMEWVLLSGTPATCANIALHNLYPGQIDLVISGPNHGRNSSTAFALSSGTLGAALAASLSVPVPGPLTSPSLHENHMPCIAISYGVVTRPVPDRILELASETAVDVCQQLFDNWGEDKEVGGKGLVPIYSINIPLVEAALEKNERKIVSTEMWRNAYGRLFKTTKLSKASYDPGDDPVQIVHGNMTSQDKPNTVQASASLPSHPHTSKTSTAGPAALPTPAPPSPIVPKETKEEDQQLKFHFAPNMHPLLFPPEGSVPEGTDAWAFAKGWISVTPMRAEYACLGATSME
Expression Data
NotationConditionNormalized Raw Expression
AYPD 30°C, concentration: 2x 10^8 cells/ml3585
BYPD 30°C, concentration: 5x10^7 cells/ml3209
CYPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml3300
DYPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml2869
EYPD 37°C, concentration: 5x10^7 cells/ml3155
FYP+galactose 30°C, concentration: 2x10^8 cells/ml3107
Gene Disruption Strategy
Mutant construction design Mutant construction design Mutant construction design Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_00146 5' flanking region primer 1AACAGGATTGCGACTTACC
L2CNAG_00146 5' flanking region primer 2TCACTGGCCGTCGTTTTACTCGGGGCACATTTCTCACAC
R1CNAG_00146 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGTCCCCAATCGTCCCTAAAG
R2CNAG_00146 3' flanking region primer 2CACGCTGTTCCTGTATTCG
SOCNAG_00146 diagnostic screening primer, pairing with B79ATCAAATGGAGGGAATCGC
POCNAG_00146 Southern blot probe primerAATGCTGTTGAGGAGTTACG
STMNAT#5 STM primerTGCTAGAGGGCGGGAGAGTT
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
* Red marked sequences indicate the extended M13 forward and reversed sequences to overlap PCR with pNAT plasmid.
Phenotype Assays
Growth
Growth phenotype
Mating Data
Mating phenotype
Virulence Factor Production
Capsule
Capsule phenotype
Urease
Urease phenotype
Melanin
Melanin phenotype
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype
Oxidative Stress
Oxidative stress phenotype
Heavy Metal Stress
Heavy metal stress phenotype
Cell Wall Stress
Cell wall stress phenotype
ER Stress
ER stress phenotype
Genotoxic Stress
Genotoxic stress phenotype
Antifungal Drug Susceptibility
Antifungal drug susceptibility
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data