Phosphatase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_06499T0
SpeciesCryptococcus neoformans var. grubii H99
Gene Annotationhypothetical protein
Gene SymbolDPP101
Phosphatase NameN/A
Phosphatase ClassLipid phosphatase
KEGG Linkout KEGG::CNN02240 
Genome Browser JBrowse link goes here.
Protein Domain / KEGG Annotation
KEGG Result
KO AccessionE-valueScore
K186932.3e-89298.2
Pfam Result
Pfam AccessionE-valueScore
PF01569 Pfam 5.5e-28 96.2
Amino Acid SequenceMALSNPFAKRADRPITTSPAGSRRRRIFISYAPDWVVTILLWGLFYLLDKINGYRRLFSVTDTSLAHPYADPERVPVWLLAVLCGIVPAVFIILVAAFVRRSFWDGHNGILGLILGLGLTATFTNIVKITVGRPRPDLFARCILPPDLTSNPVHGLTSWTVCTTTDDGRLNEGFRSFPSGHSSFAWCGMWYLILYLAAKMEINNRQGFTYKSWLLLAPLSCATLVAVSRTMDYRHHATDVIAGAVIGLLGGWYAYRQYYPPLSHPLAYKPYSPRIPKSDPPPIPLHSHSRPRSRPSTEAMLRRHESQPSQAQFHFGGESSTSPVETLSERGAGAGYGPYTADGPDGPGGIVDMNGWDRQSHTLQQPQPQKQLQTQQTSITTKGPEIQSDRQRQEQGYTPQSNNNHLQGSMLAGSGGEGVEMVERTS
Expression Data
NotationConditionNormalized Raw Expression
AYPD 30°C, concentration: 2x 10^8 cells/ml1321
BYPD 30°C, concentration: 5x10^7 cells/ml1781
CYPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml2101
DYPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml1403
EYPD 37°C, concentration: 5x10^7 cells/ml1319
FYP+galactose 30°C, concentration: 2x10^8 cells/ml1417
Gene Disruption Strategy
Mutant construction design Mutant construction design Mutant construction design Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_06499 5' flanking region primer 1AAGGGATAGAGTCGGTTGG
L2CNAG_06499 5' flanking region primer 2TCACTGGCCGTCGTTTTAC TGGCGAAAGGGTTGGATAG
R1CNAG_06499 3' flanking region primer 1CATGGTCATAGCTGTTTCCTG TTTTGGGGTCGTAAACCG
R2CNAG_06499 3' flanking region primer 2TTTCTCCGCCATTCTTCG
SOCNAG_06499 diagnostic screening primer, pairing with B79ATCGCATTTCAAGGGTGG
PO1CNAG_06499 Southern blot probe primer 1TCTAAATCCGTTTCAACCCC
PO2CNAG_06499 Southern blot probe primer 2ACGATTCCACAGAGCACAG
STMNAT#290 STM primerACCGACAGCTCGAACAAGCAAGAG
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
* Red marked sequences indicate the extended M13 forward and reversed sequences to overlap PCR with pNAT plasmid.
Phenotype Assays
Growth
Growth phenotype
Mating Data
Mating phenotype
Virulence Factor Production
Capsule
Capsule phenotype
Urease
Urease phenotype
Melanin
Melanin phenotype
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype
Oxidative Stress
Oxidative stress phenotype
Heavy Metal Stress
Heavy metal stress phenotype
Cell Wall Stress
Cell wall stress phenotype
ER Stress
ER stress phenotype
Genotoxic Stress
Genotoxic stress phenotype
Antifungal Drug Susceptibility
Antifungal drug susceptibility
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data