Detailed Locus Information for CNAG_06549T0
| Species | Cryptococcus neoformans var. grubii H99 | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | mRNA-capping enzyme subunit beta | ||||||||||||||||||
| Gene Symbol | CET1 | ||||||||||||||||||
| Phosphatase Name | N/A | ||||||||||||||||||
| Phosphatase Class | Thiamine triphosphatase | ||||||||||||||||||
| KEGG Linkout | KEGG::CNF04680 | ||||||||||||||||||
| Genome Browser | JBrowse link goes here. | ||||||||||||||||||
| Protein Domain / KEGG Annotation |
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| Amino Acid Sequence | MTTYIPIHDRPPHYANLPSPPSNSGQLRPLRYSSPDIDPDTPLPRMSSTHESDPPEQINTTHSGNHAPRQRVGSQGAEQEGQSRKRARISPSHLGGNGSYVPRQRTEKSHHPHHDDHSGHATSDQHGNGQVYRPRHGPAPLTGSIFNLSPRNPFTSVVGDFIMNAAMGHSNVEIELKLGTFMTPSMPGQQPRRINMPTLSEMIIPHDYPNGPFVSTINHLHHRALNELLNRAVESQSTHPTGRLYFSRSKLADSFYDHSEHGRGKVRVSRDMDNGHVVQAVEKRRIADMNVYCPGMAYDFRISVNTETPCEVPTGNAKSVRYKDRACYRHQVCRVDLTSVFSSNPRNADVPPSRSFELEIEVLDVPALLAEGAAQSERFDEILQNVLDSARMLVKNI | ||||||||||||||||||
Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 1734 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 2442 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 2125 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 2448 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 2437 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 1784 |
Gene Disruption Strategy
| Primer Name | Description | Primer Sequence |
|---|---|---|
| L1 | CNAG_06549 5' flanking region primer 1 | TCCAAACAAACACTCCAGC |
| L2 | CNAG_06549 5' flanking region primer 2 | TCACTGGCCGTCGTTTTACAGATTGGCGTAATGAGGCG |
| R1 | CNAG_06549 3' flanking region primer 1 | CATGGTCATAGCTGTTTCCTGAAGCGAACGGTTTGACGAG |
| R2 | CNAG_06549 3' flanking region primer 2 | CGAAAAGAAGGGTGTGACG |
| SO | CNAG_06549 diagnostic screening primer, pairing with B79 | CCATCACAAGTCCTTCTTTGG |
| PO | CNAG_06549 Southern blot probe primer | ACTGCCATTCCCTCCTAAG |
| STM | NAT#225 STM primer | CCATAGAACTAGCTAAAGCA |
| STM common | STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
* Red marked sequences indicate the extended M13 forward and reversed sequences to overlap PCR with pNAT plasmid.