Detailed Locus Information for CNAG_06617T0
| Species | Cryptococcus neoformans var. grubii H99 | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | hypothetical protein | ||||||||||||||||||
| Gene Symbol | Unknown | ||||||||||||||||||
| Phosphatase Name | N/A | ||||||||||||||||||
| Phosphatase Class | Phosphoprotein phosphatase | ||||||||||||||||||
| KEGG Linkout | KEGG::CNF04020 | ||||||||||||||||||
| Genome Browser | JBrowse link goes here. | ||||||||||||||||||
| Protein Domain / KEGG Annotation |
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| Amino Acid Sequence | MRTIPLLCFNDVYRVNQKYNPQPGAPEDNSSDRTITVSQFAELLLSERSKWANRQIEDQDDQEGASKEGLVLFAGDVFNPSVESSVTRGSHMVPIMNALKVDYACVGNHDFDFGFPHLTKLIESTSFPWLLSNIVDCNTGRQPEPLKRFIVTERCGVKIGLIGLVEKDWIATIPSWPHNFKYRSMKDTALELSRELRDPNGEHQVDIIIALTHCRVPNDIRLATELGAVADKPGVQNEHGIDLIIGGHDHIYYIGKGATSWEGYAGRKDVPGTTEDHGVRLIKSGTDFRDLTSANLTVTPAPLGSIRRQLITSLTGKHLYVLPSSPSSPPFEELVKSLLSSVSEALTKPVCFTLTPFDARSEVVRTQESGLGNWIADVLRHAYAESIVQAKDKEGGLGDEYEGDADAAILCGGTLRGDSQYGPGKISLGDILEILPFDDPVVCIELDGKGIWNALESALSKWPAQEGRFPIVSGLAVKWDHTRPPGQRILSVHQLAQPKKDDDDWEDPADMVDFKEQEDGTTVVVKQKKLQLGEEVKNEEGGRMYKVITRDYMAQGYDGFEALKNRNFIVDDENGQIMSSILRSFLLGSSYIFRHKQLEEAAHSHLSRRTSQVLSRARAEHTSQSQSSPSASLSSSPKRNLLAAQFKSPQDRMTSPNHLTSASLPPNALSPNSEISESSAWGRLRRHVVQHDWGTIRNALHVAKHEHMSGLDVLAGQAMRQARNHMPGAWSPIQTPPTQERPQYDGIVLPKDDGGEANLADLAIVSPLVDGRMRDISAGKE | ||||||||||||||||||
Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 3607 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 4011 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 4392 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 3293 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 3333 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 3399 |
Gene Disruption Strategy
| Primer Name | Description | Primer Sequence |
|---|---|---|
| L1 | CNAG_06617 5' flanking region primer 1 | ATTCAACCTTCCTTTGGGC |
| L2 | CNAG_06617 5' flanking region primer 2 | TCACTGGCCGTCGTTTTACTTGAAGCAGAGCAATGGG |
| R1 | CNAG_06617 3' flanking region primer 1 | CATGGTCATAGCTGTTTCCTGGGTGGATGGAAGGATGAGAG |
| R2 | CNAG_06617 3' flanking region primer 2 | TCTCGGTCACCTTCTTACG |
| SO | CNAG_06617 diagnostic screening primer, pairing with B79 | CTGAGAGAAAGGAACGGTG |
| PO | CNAG_06617 Southern blot probe primer | CCAATCCTTCTTTGCTCGC |
| STM | NAT#273 STM primer | GAGATCTTTCGGGAGGTCTGGATT |
| STM common | STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
* Red marked sequences indicate the extended M13 forward and reversed sequences to overlap PCR with pNAT plasmid.