Detailed Locus Information for CNAG_07354T0
| Species | Cryptococcus neoformans var. grubii H99 | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | 3'(2'),5'-bisphosphate nucleotidase | ||||||||||||||||||
| Gene Symbol | MET22 | ||||||||||||||||||
| Phosphatase Name | N/A | ||||||||||||||||||
| Phosphatase Class | Lipid phosphatase | ||||||||||||||||||
| KEGG Linkout | KEGG::CNA03210 | ||||||||||||||||||
| Genome Browser | JBrowse link goes here. | ||||||||||||||||||
| Protein Domain / KEGG Annotation |
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| Amino Acid Sequence | MATQLPFTRLVKETQIGILSVLRACYLTKNVQDTLVTKDTLLKSDKSPVTVADLSAQSLISLHLLAHFQDPIIGEEDTSELRVNEPLRQRVIGLVNGGFEKEEGWGKDKTFSEEDILSAIDAGSAEGGNKGRFWTIDPVDGTSGFIRHQQYAVCLALIVDGVVELGVIGCPNLGPEPAKIGEEIIPNGKGVLMVAVRGEGSWSRPLDSATYTKLNLPPSPPASNPLTFLESVESGHSAHSVQARIGSLLGVQRPSLRMDSQAKYTCLSRGEGGVYLRIPTKYVGGKVYEERIWDHAPGALLIHESGGICTDMWGKELNFGVGRTLKENDGIVAAGKDIHPKAVEAVKKAIEEAQAEKK | ||||||||||||||||||
Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 3598 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 7009 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 6604 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 7363 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 4468 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 3553 |
Gene Disruption Strategy
| Primer Name | Description | Primer Sequence |
|---|---|---|
| L1 | CNAG_07354 5' flanking region primer 1 | AACCATTCGTCAGGCTTG |
| L2 | CNAG_07354 5' flanking region primer 2 | TCACTGGCCGTCGTTTTACAGTGAAGGGGAGTTGAGTGG |
| R1 | CNAG_07354 3' flanking region primer 1 | CATGGTCATAGCTGTTTCCTGCGCAGGCTGAAAAGAAGTG |
| R2 | CNAG_07354 3' flanking region primer 2 | TTATCGGTTTGGAGGGGGAG |
| SO | CNAG_07354 diagnostic screening primer, pairing with B79 | CGGGAAATCACAACTTACACC |
| PO | CNAG_07354 Southern blot probe primer | AAAGGTCTGCGACTGTCAC |
| STM | NAT#218 STM primer | CTCCACATCCATCGCTCCAA |
| STM common | STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
* Red marked sequences indicate the extended M13 forward and reversed sequences to overlap PCR with pNAT plasmid.