Detailed Locus Information for CNAG_07434T0
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | HAD hydrolase, family IIID | |||||||||
| Gene Symbol | HAD3 | |||||||||
| Phosphatase Name | N/A | |||||||||
| Phosphatase Class | Haloacid dehalogenase | |||||||||
| KEGG Linkout | KEGG::CND03300 | |||||||||
| Genome Browser | JBrowse link goes here. | |||||||||
| Protein Domain / KEGG Annotation |
|
|||||||||
| Amino Acid Sequence | MSQDEPTGRRSPSCSEPHLHTDTTGNNISTQDSLSEDLEEGMKTVNARSDAETAASGKRVKVFHTSNTEEEQARRHKAFGNEDVMAREEETGAEEKEKSTNDEDASTTAFSAIINQRDQGDGHLADSDNVNMDNGDDESPQVSTVPLAEETDEEWWDLKMQWGGKVYDIHVGGNDMVYDFREKIASLTSIPPDAQKLIGLSPTVKGKLNASHDAMRFAHLGVKKGGKFVLVGTKVEERFVDPIKEQEGEGGEGEFDVDYKGIGRGNDPRNKRKIQEIIEKVPITVMNAPREGKKLLVLDLDYTIVDTKPLLSGTLPSSECARPGLHDFLELVYPHYDIVIWSQTSWRWLETKLIELDLFSDSRGYKISFVIDRTCMFPELKVFSQRNGQPYKHEVKPLAYLWASFPQWSAKNTIHIDDLSRNFALNPGEGLKIRAFNKAGSPDGRRDRELIKLGTYLIGIAASQRDFTTVNHKYWNGRVKGRRGRH | |||||||||
Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 2738 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 2407 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 2537 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 1785 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 1501 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 1884 |
Gene Disruption Strategy
| Primer Name | Description | Primer Sequence |
|---|---|---|
| L1 | CNAG_07434 5' flanking region primer 1 | CCCATCTCGTTTTCAATCTGTG |
| L2 | CNAG_07434 5' flanking region primer 2 | TCACTGGCCGTCGTTTTACTCGCTACACGATGGTGAAC |
| R1 | CNAG_07434 3' flanking region primer 1 | CATGGTCATAGCTGTTTCCTGATGGGAGGGTAAAGGGAAG |
| R2 | CNAG_07434 3' flanking region primer 2 | GACACGCAAGGAGGTAAAG |
| SO | CNAG_07434 diagnostic screening primer, pairing with B79 | GCTTAGTCCTTGTAACCTGAG |
| PO | CNAG_07434 Southern blot probe primer | CCTCTGTGTTGGAAGTATGG |
| STM | NAT#116 STM primer | GCACCCAAGAGCTCCATCTC |
| STM common | STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
* Red marked sequences indicate the extended M13 forward and reversed sequences to overlap PCR with pNAT plasmid.
Phenotype Assays
| Growth | |
|---|---|
 | |
| Mating Data | |
 | |
| Virulence Factor Production | |
|
Capsule  Urease  Melanin  | |
| Stress Response and Adaptation | |
|
Osmotic Stress  Oxidative Stress  Heavy Metal Stress  Cell Wall Stress  ER Stress  Genotoxic Stress  | |
| Antifungal Drug Susceptibility | |
 | |
| Virulence Data by the Insect Host Model (Galleria mellonella) | |
 | |
| Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
 |