Phosphatase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_07547T0
SpeciesCryptococcus neoformans var. grubii H99
Gene Annotationhypothetical protein
Gene SymbolGPM1
Phosphatase NameN/A
Phosphatase ClassHistidine phosphatase
KEGG Linkout KEGG::CNK01230 
Genome Browser JBrowse link goes here.
Protein Domain / KEGG Annotation
KEGG Result
KO AccessionE-valueScore
K146347.9e-37117.5
Pfam Result
Pfam AccessionE-valueScore
PF00300 Pfam 6.9e-33 112.2
Amino Acid SequenceMNRNFTSLSRQHTRRAGNMITLTIVRHGESTDNLKPLWAGWSDAPLSQHGMNQAKALGESLKDTKFDYIFASDLKRAHWTSQQILKNQAEPKPPLVVSELLREQHFGEGEQKPFGENSGWVRQPGRVFKFPGGESLTDVRKRADQAVEQFIEPILKECRGKPATSKHVVVVAHGIFNSEFLGALMARRKTNAPLEWNYRGMTNTGWTKAEVGYADEARTETTTQLSTDPSGPVSPSSAPSAPPPSTPNPDNLEPLVIKVLCSDVTDHLKGVIRQKGGLGSQSYDEKQEDIRKFFGGGGAK
Expression Data
NotationConditionNormalized Raw Expression
AYPD 30°C, concentration: 2x 10^8 cells/ml10943
BYPD 30°C, concentration: 5x10^7 cells/ml4477
CYPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml3999
DYPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml3859
EYPD 37°C, concentration: 5x10^7 cells/ml5288
FYP+galactose 30°C, concentration: 2x10^8 cells/ml4555
Gene Disruption Strategy
Mutant construction design Mutant construction design Mutant construction design Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_07547 5' flanking region primer 1CATTCGCTTGTCTTGACTG
L2CNAG_07547 5' flanking region primer 2TCACTGGCCGTCGTTTTACTTGTCAGTTCAAGCGGAC
R1CNAG_07547 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGCCTGAAAGGGGTGATTAGAC
R2CNAG_07547 3' flanking region primer 2TTAGTCGGGTAGAAGGGACAGG
SOCNAG_07547 diagnostic screening primer, pairing with B79GGAAGGTTTATGGAGTGGTAG
POCNAG_07547 Southern blot probe primerGGTCAGACTTTCTCCTCCTG
STMNAT#219 STM primerCCCTAAAACCCTACAGCAAT
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
* Red marked sequences indicate the extended M13 forward and reversed sequences to overlap PCR with pNAT plasmid.
Phenotype Assays
Growth
Growth phenotype
Mating Data
Mating phenotype
Virulence Factor Production
Capsule
Capsule phenotype
Urease
Urease phenotype
Melanin
Melanin phenotype
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype
Oxidative Stress
Oxidative stress phenotype
Heavy Metal Stress
Heavy metal stress phenotype
Cell Wall Stress
Cell wall stress phenotype
ER Stress
ER stress phenotype
Genotoxic Stress
Genotoxic stress phenotype
Antifungal Drug Susceptibility
Antifungal drug susceptibility
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data