Phosphatase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_07740T0
SpeciesCryptococcus neoformans var. grubii H99
Gene Annotationhypothetical protein
Gene SymbolHAD2
Phosphatase NameN/A
Phosphatase ClassHaloacid dehalogenase
KEGG Linkout KEGG::CNG00790 
Genome Browser JBrowse link goes here.
Protein Domain / KEGG Annotation
KEGG Result
KO AccessionE-valueScore
K089667.8e-46155.3
Pfam Result
Pfam AccessionE-valueScore
PF12710 Pfam 1.1e-22 79.7
Amino Acid SequenceMSPAAAPESVLPYPPIKKDAKFVVLSDWDGTITDKDSNDFLTDKLGFGFEKRRALNLEVLDGRISFRDSFRQMLESIKVPFEECKEELMKNIKLDPGFEKFYIWCKQNGVPVVIVSSGMTPNIRGVLSTLLPGPDAEEIDIIANDVKFTDPEGKGNTWEIVFRHPTSGFGHDKSQAILPYRDLPHKPTLFFCGDGVSDLSAAKHADLLFAKTMVNGHSDLQTYCEKQGIRHVPFVDFTKVLEKVQEVVGGKSVDEVLKEEEGK
Expression Data
NotationConditionNormalized Raw Expression
AYPD 30°C, concentration: 2x 10^8 cells/ml4142
BYPD 30°C, concentration: 5x10^7 cells/ml15105
CYPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml15097
DYPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml15677
EYPD 37°C, concentration: 5x10^7 cells/ml11966
FYP+galactose 30°C, concentration: 2x10^8 cells/ml3999
Gene Disruption Strategy
Mutant construction design Mutant construction design Mutant construction design Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_07740 5' flanking region primer 1ATGGAAGTGGGGAGGTTAC
L2CNAG_07740 5' flanking region primer 2TCACTGGCCGTCGTTTTACGTATGGCAAGACGGATTCG
R1CNAG_07740 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGTGTGGACGAGGTTCTCAAG
R2CNAG_07740 3' flanking region primer 2TTGAGATGTTGAGGAGGGG
SOCNAG_07740 diagnostic screening primer, pairing with B79GGAAGGACGAAGGGATTTG
POCNAG_07740 Southern blot probe primerTCGTTACTGTCCTTGTCGG
STMNAT#123 STM primerCTATCGACCAACCAACACAG
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
* Red marked sequences indicate the extended M13 forward and reversed sequences to overlap PCR with pNAT plasmid.
Phenotype Assays
Growth
Growth phenotype
Mating Data
Mating phenotype
Virulence Factor Production
Capsule
Capsule phenotype
Urease
Urease phenotype
Melanin
Melanin phenotype
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype
Oxidative Stress
Oxidative stress phenotype
Heavy Metal Stress
Heavy metal stress phenotype
Cell Wall Stress
Cell wall stress phenotype
ER Stress
ER stress phenotype
Genotoxic Stress
Genotoxic stress phenotype
Antifungal Drug Susceptibility
Antifungal drug susceptibility
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data