Detailed Locus Information for CNAG_00182T1
| Species | Cryptococcus neoformans var. grubii H99 | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | retrograde transporter | ||||||||||||||||||
| Gene Symbol | VPS29 | ||||||||||||||||||
| Phosphatase Name | N/A | ||||||||||||||||||
| Phosphatase Class | Phosphoprotein phosphatase | ||||||||||||||||||
| KEGG Linkout | KEGG::CNA01710 | ||||||||||||||||||
| Genome Browser | JBrowse link goes here. | ||||||||||||||||||
| Protein Domain / KEGG Annotation |
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| Amino Acid Sequence | MVLVLVIGDLHIPNLVHDLPAKFKKLLVPGKIGQIICTGNVCDKETYDYLRTTAPEVHVVRGEFDENPHFPLSLIIQHQSLRIGVVHGQQVVPAGDPDMLAALARQMDVDVLISGGTHRFESFEFEGRFFVNPGSATGAWSSLWNGEVTPSFALMDIQGPVIVTYVYQLVDGEVKVDKVEYRKPDLTSETQSQSTRSEVAARW | ||||||||||||||||||
Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 2981 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 4361 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 4839 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 3869 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 3778 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 2937 |
Gene Disruption Strategy
| Primer Name | Description | Primer Sequence |
|---|---|---|
| L1 | CNAG_00182 5' flanking region primer 1 | TGTCTTCTTGAAAGGGGTTG |
| L2 | CNAG_00182 5' flanking region primer 2 | TCACTGGCCGTCGTTTTACAATGACGAGAACCAGGACC |
| R1 | CNAG_00182 3' flanking region primer 1 | CATGGTCATAGCTGTTTCCTGACTCAGTCTCAATCAACGC |
| R2 | CNAG_00182 3' flanking region primer 2 | GTTGTTTCTTCTTTCCCAGC |
| SO | CNAG_00182 diagnostic screening primer, pairing with B79 | AATCAGGCGTGGGTTCAGAC |
| PO | CNAG_00182 Southern blot probe primer | ATAGGTCTCCTTGTCGCAG |
| STM | NAT#295 STM primer | ACACCTACATCAAACCCTCCC |
| STM common | STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
* Red marked sequences indicate the extended M13 forward and reversed sequences to overlap PCR with pNAT plasmid.
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule  Urease  Melanin  | |
| Stress Response and Adaptation | |
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Osmotic Stress  Oxidative Stress  Heavy Metal Stress  Cell Wall Stress  ER Stress  Genotoxic Stress  | |
| Antifungal Drug Susceptibility | |
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| Virulence Data by the Insect Host Model (Galleria mellonella) | |
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| Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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