Phosphatase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_00408T0
SpeciesCryptococcus neoformans var. grubii H99
Gene Annotationserine/threonine-protein phosphatase PP-X isozyme 1
Gene SymbolPPH3
Phosphatase NameN/A
Phosphatase ClassPhosphoprotein phosphatase
KEGG Linkout KEGG::CNA03970 
Genome Browser JBrowse link goes here.
Protein Domain / KEGG Annotation
KEGG Result
KO AccessionE-valueScore
K154231.1e-176585.5
Pfam Result
Pfam AccessionE-valueScore
PF00149 Pfam 4.4e-46 156.1
Amino Acid SequenceMSLSSDLDKQIEQLKRCETIPESAVKELCTKAKEILMEEGNVQYVDSPVTICGDIHGQFFDLMELFKIAGFCPETNYIFMGDFVDRGFYSVETFLLLLLLKVRYPDRITLIRGNHESRQITQVYGFYDECQRKYGSSNVWRYCCDVFDYLSLGCVVDGRVFCVHGGLSPAVHRLDQIRVIDRRQEVPHEGPMCDLLWSDPDEEIQGWGMSPRGAGFLFGRDVVEQFNHANDIELVARAHQLVMEGYKLMFDRRIVTVWSAPNYCYRCGNTASVLELDENLRQEYKTFDAAPPDARSIPQKRPMTHEYFL
Expression Data
NotationConditionNormalized Raw Expression
AYPD 30°C, concentration: 2x 10^8 cells/ml2051
BYPD 30°C, concentration: 5x10^7 cells/ml2183
CYPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml2234
DYPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml2029
EYPD 37°C, concentration: 5x10^7 cells/ml2299
FYP+galactose 30°C, concentration: 2x10^8 cells/ml2180
Gene Disruption Strategy
Mutant construction design Mutant construction design Mutant construction design Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_00408 5' flanking region primer 1CCAACCTTTTTCTCACCC
L2CNAG_00408 5' flanking region primer 2TCACTGGCCGTCGTTTTACTTGGAGAGTTTGTGGACG
R1CNAG_00408 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGCCGCAAAAACGACCTATG
R2CNAG_00408 3' flanking region primer 2CGAGTGGCTGTTCTCTAATC
SOCNAG_00408 diagnostic screening primer, pairing with B79AAAGCCTTCTCGTAGTCGG
POCNAG_00408 Southern blot probe primerCAGAGTAGAATCCTCGGTCG
STMNAT#146 STM primerACTAGCCCCCCCTCACCACCT
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
* Red marked sequences indicate the extended M13 forward and reversed sequences to overlap PCR with pNAT plasmid.
Phenotype Assays
Growth
Growth phenotype
Mating Data
Mating phenotype
Virulence Factor Production
Capsule
Capsule phenotype
Urease
Urease phenotype
Melanin
Melanin phenotype
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype
Oxidative Stress
Oxidative stress phenotype
Heavy Metal Stress
Heavy metal stress phenotype
Cell Wall Stress
Cell wall stress phenotype
ER Stress
ER stress phenotype
Genotoxic Stress
Genotoxic stress phenotype
Antifungal Drug Susceptibility
Antifungal drug susceptibility
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data