Phosphatase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_00498T0
SpeciesCryptococcus neoformans var. grubii H99
Gene Annotationcell division cycle protein 14
Gene SymbolCDC14
Phosphatase NameN/A
Phosphatase ClassProtein tyrosine phosphatase
KEGG Linkout KEGG::CNA04770 
Genome Browser JBrowse link goes here.
Protein Domain / KEGG Annotation
KEGG Result
KO AccessionE-valueScore
K066391.4e-125418.6
Pfam Result
Pfam AccessionE-valueScore
PF14671 Pfam 2.3e-30 99
Amino Acid SequenceMPSTSTRAFPNEVAVFSNYLLFTTLTFKEVQAHAKTPYGHPGNKRACSLFTLDDDMRYTSFAMDHGPLNLAFTFHACIRIHEKLEKARERGKPVCLYTTTEPKMKSNMILIAALYSLIVDKQPPWNAFRPIAQFEVMPFRDAGSGPMDYGLTVQDILYGVEKAVGNGLLDLASFDADEYQYYEMVENGDLNILGPFIPFASPTENSWIEGVLQSPPNGRILHTPVKSRTISHQLRCVLDIFERENVGLVARLNDELYDRRHFLDMGIEHIEMFFDDGTNPPDDIVREFIRLAEHTIEHKRQKVAVHCKAGLGRTGVLIGAYLVYKYQFTAQEAIGFMRIVRPGMVVGPQQQYMVLNQLKWAGWAARDQALKEIAQASCMESNPPIAPPMEIVIKPYAGQDIETSTHIPPITRSPKLRPRRSQSSSSLATISPRRGGDAVGQPRKTRQLVEPTTETEHEELRSSQSHLSPSNPLVVREVVLEAPADQKENGVNPSTVSPLGSIRGTKRSATRPSPGYEHSNSLPRVSLNVPSSSLTRANSNVSIGSEGSVDERPPKRRTGSSPPEVEESGATVSPVSEPMVAEQVETPVRLTATKNLRLRTHSSSPSPPPASTPSVPGTTPTRVNLAASTEAPSTPTRAPSRVIPENTNVIASVRRTMPAKKSFLPVRKNVESKSTPVRATTNPATAQSPPLSTRTSARVANTLNRYEDIAVNSNGNAKNVKTATPASRKLGRGVTKKGVLTPPRITEMWGQLSRIGSSPPADKEKQ
Expression Data
NotationConditionNormalized Raw Expression
AYPD 30°C, concentration: 2x 10^8 cells/ml1588
BYPD 30°C, concentration: 5x10^7 cells/ml1432
CYPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml1697
DYPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml1626
EYPD 37°C, concentration: 5x10^7 cells/ml1874
FYP+galactose 30°C, concentration: 2x10^8 cells/ml2087
Gene Disruption Strategy
Mutant construction design Mutant construction design Mutant construction design Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_00498 5' flanking region primer 1GGCAATGGAGTAGGGAATC
L2CNAG_00498 5' flanking region primer 2TCACTGGCCGTCGTTTTACGCTTACAAGTTCATCGCCTC
R1CNAG_00498 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGCGTCAACTTTTGCTGGATTTGG
R2CNAG_00498 3' flanking region primer 2CAAATCTCGTCTTCAGAGTGC
SOCNAG_00498 diagnostic screening primer, pairing with B79GGACACAAGTATTATCTACCGC
POCNAG_00498 Southern blot probe primerGATGGATGATAATGGTGGTG
STMNAT#116 STM primerGCACCCAAGAGCTCCATCTC
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
* Red marked sequences indicate the extended M13 forward and reversed sequences to overlap PCR with pNAT plasmid.
Phenotype Assays
Growth
Growth phenotype
Mating Data
Mating phenotype
Virulence Factor Production
Capsule
Capsule phenotype
Urease
Urease phenotype
Melanin
Melanin phenotype
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype
Oxidative Stress
Oxidative stress phenotype
Heavy Metal Stress
Heavy metal stress phenotype
Cell Wall Stress
Cell wall stress phenotype
ER Stress
ER stress phenotype
Genotoxic Stress
Genotoxic stress phenotype
Antifungal Drug Susceptibility
Antifungal drug susceptibility
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data