Detailed Locus Information for CNAG_00533T0
| Species | Cryptococcus neoformans var. grubii H99 | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | mitochondrial import inner membrane translocase subunit TIM50 | ||||||||||||||||||
| Gene Symbol | TIM50 | ||||||||||||||||||
| Phosphatase Name | N/A | ||||||||||||||||||
| Phosphatase Class | Haloacid dehalogenase | ||||||||||||||||||
| KEGG Linkout | KEGG::CNA05150 | ||||||||||||||||||
| Genome Browser | JBrowse link goes here. | ||||||||||||||||||
| Protein Domain / KEGG Annotation |
|
||||||||||||||||||
| Amino Acid Sequence | MLRQATSRFLSSTHRSIRPLSTSAPSFIRIRSQASEPSPAERPPPVPENVNPSQPFEPEVNKSEGATKAAETQQAEGAASAGTPLTPPQPEVVFGNTRSAASTTPEMEATVENPDYSKLPSLDIDPEAAAIPEPATGKDQEAEGEKKKTGAGKKEYVSSQEKSRRMWIRAGYGALAVGAVGAVLAMGSEETTGKKQGGFVETFQNNMLELFDFFNKPAFQTLLPDPLPPPHQRPYTLCIDLEGLLVHSSWDRTHGWRTAKRPGVDYFLGYLSQFYEIVLFSSQPLYTAAPIAEKIDPYQAFMPYRLFRESTRSVKGKVVKDISFLNRDPSKVIVLDVNPEHVALQPENGIVLQPWDGSPRDKGLVDMIPFLESIGIFNPADVRPILQAYAGKDIPIEYAKKEAEAKAKAIEEWERTHPTAITGAGSGFLSSIFGSVAAPGSSRPNQPMTYLEQKRAQAQRIYQEEQKYWAEHADEFKKLIEEDKQRQLAEMKGSILGYLGAPKMQDGPKEDVLKA | ||||||||||||||||||
Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 10564 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 9438 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 8871 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 10795 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 9274 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 11245 |
Gene Disruption Strategy
| Primer Name | Description | Primer Sequence |
|---|---|---|
| L1 | CNAG_00533 5' flanking region primer 1 | TGGTAGAACGAGGAGCATC |
| L2 | CNAG_00533 5' flanking region primer 2 | TCACTGGCCGTCGTTTTACGAATAGAACGGTGAGTAGACG |
| R1 | CNAG_00533 3' flanking region primer 1 | CATGGTCATAGCTGTTTCCTGGGTTCCATCTTGGGTTATTTGG |
| R2 | CNAG_00533 3' flanking region primer 2 | TTTACTTCCGCCGCATAC |
| SO | CNAG_00533 diagnostic screening primer, pairing with B79 | CCTATCCAGAAACTCAACAGAC |
| PO1 | CNAG_00533 Southern blot probe primer 1 | CCAGGTATTCTTCCGTTGG |
| PO2 | CNAG_00533 Southern blot probe primer 2 | GCTCACATACTCCTTTTTACCG |
| STM | NAT#6 STM primer | ATAGCTACCACACGATAGCT |
| STM common | STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
* Red marked sequences indicate the extended M13 forward and reversed sequences to overlap PCR with pNAT plasmid.