Phosphatase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_00554T0
SpeciesCryptococcus neoformans var. grubii H99
Gene Annotationinositol phosphorylsphingolipid-phospholipase C
Gene SymbolISC1
Phosphatase NameN/A
Phosphatase ClassEndonuclease exonuclease phosphatase
KEGG Linkout KEGG::CNA05370 
Genome Browser JBrowse link goes here.
Protein Domain / KEGG Annotation
KEGG Result
KO AccessionE-valueScore
K123513.7e-112373.7
Pfam Result
Pfam AccessionE-valueScore
PF03372 Pfam 3e-26 90.8
Amino Acid SequenceMNSQSPPEDPDLTLKVLSFNVWGLAIVAKNRHTRIHAIAEYLASSNYDIVCLQELWIYKDYEVVREEVQRNLPFSRFFHTGALGSGLAIFTRFPLIAAHALPYSLSGSPAQAFAGDFFVKKAAANVVILHPVLGEVEIWNTHMHAAGEHPPDTRQAHRIAQSWQLANAVRGGAAKGRYVFVMGDFNSQPWSIPIAMMRDHAQLMDSFDQVHPSANSEISPPPSPAEALKVYGMTCDSPLNTYSAGKPIPEHVLEKGGKRLDYIFFRQPAIARRRPLLWKYKDETDGEENHWGNNKSDQGHIESGKPLQDSVSSAPSLRCVDSQVILTNRVPGHLHSYSDHFALFSTFVIDTPGKKKHTDNANPQDESSNLSHDNSSEPLMPILSQSKADPIGSNVYAVRDPFKNHSRSSNPSSPRTSTSSIPKASTIRSALGTLRLYTRISSHTSTSHLRICGVMLLTLVGLTVGSAWQPKSWLQPIFTILAGLLGAAAVTFLYTGFIWGRWEVGLLTEVTEEMELELRVVEMDENVHR
Expression Data
NotationConditionNormalized Raw Expression
AYPD 30°C, concentration: 2x 10^8 cells/ml7832
BYPD 30°C, concentration: 5x10^7 cells/ml5927
CYPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml6712
DYPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml3375
EYPD 37°C, concentration: 5x10^7 cells/ml2718
FYP+galactose 30°C, concentration: 2x10^8 cells/ml6172
Gene Disruption Strategy
Mutant construction design Mutant construction design Mutant construction design Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_00554 5' flanking region primer 1ATCTCCCCATCTCTTCACC
L2CNAG_00554 5' flanking region primer 2TCACTGGCCGTCGTTTTACACAGGACCTTGAGAGTGAG
R1CNAG_00554 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGACTCGTTGGTCTTACCGTG
R2CNAG_00554 3' flanking region primer 2ATCGGCATCAACAATCCC
SOCNAG_00554 diagnostic screening primer, pairing with B79CGTATCATTTCTTGGCAACG
PO1CNAG_00554 Southern blot probe primer 1AGGAGTTCTACCTTCTGGTTAG
PO2CNAG_00554 Southern blot probe primer 2AACGAAAAAGTCCCCTGC
STMNAT#43 STM primerCCAGCTACCAATCACGCTAC
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
* Red marked sequences indicate the extended M13 forward and reversed sequences to overlap PCR with pNAT plasmid.
Phenotype Assays
Growth
Growth phenotype
Mating Data
Mating phenotype
Virulence Factor Production
Capsule
Capsule phenotype
Urease
Urease phenotype
Melanin
Melanin phenotype
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype
Oxidative Stress
Oxidative stress phenotype
Heavy Metal Stress
Heavy metal stress phenotype
Cell Wall Stress
Cell wall stress phenotype
ER Stress
ER stress phenotype
Genotoxic Stress
Genotoxic stress phenotype
Antifungal Drug Susceptibility
Antifungal drug susceptibility
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data