Phosphatase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_00810T0
SpeciesCryptococcus neoformans var. grubii H99
Gene Annotationpolynucleotide kinase 3'-phosphatase
Gene SymbolHAD4
Phosphatase NameN/A
Phosphatase ClassHaloacid dehalogenase
KEGG Linkout KEGG::CNA07870 
Genome Browser JBrowse link goes here.
Protein Domain / KEGG Annotation
KEGG Result
KO AccessionE-valueScore
K080731.9e-135451.9
Pfam Result
Pfam AccessionE-valueScore
PF08645 Pfam 1.2e-54 182
Amino Acid SequenceMPPQKRTAEWPEPPAKKTHPFFTNEPKEHGKFHPSDPALIHFTHLDPFESLPNPSSGTTNPIASVSSSKKIPVAFYDLDGTLIKTRSGNDFPKSRDDWMWWHPSVPEKLKQEWEDGTHLVVISNQGSKKPKIKSEWRAKLPLIAAKMPNNVPLRILAAIEQNNIYRKPNIGMFQAITEIYRARGLEIDMEKSIFVGDAAGRPAKGSRKKDHGNTDYKFAINVGLRFVTPEEHFLGHPRPSFPDPPIGFRPRNLGIFDILPHIVPSHTPITRKTDTEVEIVIFVGYPASGKSSFFRKHFQPAGYVHVNQDTLRTREKCLNVAEQAVKGGKSVVIDNTNRNRETRAYWVALASKLNVPIRLFHFLCPPELAKHNNLYRAYYPPPDEPTRGLLPYIAFAGFETAFEEPKAEEGFKEVRMVNFHWEGSEEQRKKWDMYIE
Expression Data
NotationConditionNormalized Raw Expression
AYPD 30°C, concentration: 2x 10^8 cells/ml2483
BYPD 30°C, concentration: 5x10^7 cells/ml3572
CYPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml3306
DYPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml2998
EYPD 37°C, concentration: 5x10^7 cells/ml3116
FYP+galactose 30°C, concentration: 2x10^8 cells/ml2085
Gene Disruption Strategy
Mutant construction design Mutant construction design Mutant construction design Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_00810 5' flanking region primer 1GTATTGAGAGTGGTGGTGC
L2CNAG_00810 5' flanking region primer 2TCACTGGCCGTCGTTTTACCTTTTCTGAGGGGGCATAAC
R1CNAG_00810 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGAGAGCAGAGGAAGAAGTGGG
R2CNAG_00810 3' flanking region primer 2GCCATCCATCCATCACCTTC
SOCNAG_00810 diagnostic screening primer, pairing with B79GCAAGATTACCACAGGCTC
POCNAG_00810 Southern blot probe primerATGGGATTGGTGGTTCCAG
STMNAT#116 STM primerGCACCCAAGAGCTCCATCTC
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
* Red marked sequences indicate the extended M13 forward and reversed sequences to overlap PCR with pNAT plasmid.
Phenotype Assays
Growth
Growth phenotype
Mating Data
Mating phenotype
Virulence Factor Production
Capsule
Capsule phenotype
Urease
Urease phenotype
Melanin
Melanin phenotype
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype
Oxidative Stress
Oxidative stress phenotype
Heavy Metal Stress
Heavy metal stress phenotype
Cell Wall Stress
Cell wall stress phenotype
ER Stress
ER stress phenotype
Genotoxic Stress
Genotoxic stress phenotype
Antifungal Drug Susceptibility
Antifungal drug susceptibility
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data