Phosphatase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_02681T0
SpeciesCryptococcus neoformans var. grubii H99
Gene Annotationphytase
Gene SymbolAPH3
Phosphatase NameN/A
Phosphatase ClassHistidine phosphatase
KEGG Linkout KEGG::CNK01690 
Genome Browser JBrowse link goes here.
Protein Domain / KEGG Annotation
KEGG Result
KO AccessionE-valueScore
K010786e-46156.4
Pfam Result
Pfam AccessionE-valueScore
PF00328 Pfam 6.8e-50 169
Amino Acid SequenceMIVSNDTPTPSTPLLLHVMQSPSHRSKVYIQQYRSGKRRHPLFLFLLVITPITLITLVTLLAWDTSPLGHCYIKPICRALKDGNEMEEIWWRNQGPYAPYKELDHSKNLESLPRGCEVDQVTLLHRHTSRYPTANAGRCMLGALSKIRRREIGVPRHHPELSFIDKADLELKDWHFDGLTNQGRKAAWRSGVHIAIAYKSLLDKTVDVFTRSSGGERVLETSGYWLEGFRKHRFAIKRKSDLPKVDVIIPEEPTFNSTLSVHSCPAFETLDHSPGSTAQSDLSPLLSPAIDRLNTALRPKPKLDADDVACLADMCGYDSQSRGTEWKRWSKWCGLFTKDEWEVLGHGKDLKRYYEVGQGSDYGPTMGAGYINEIIARLTDSAPQDNTSTNRTLDADEHTFPRGGERFFVDFGHDNEMLEVLAAAGVLKQPRPLATTEVPARRTFILSRIVPFGARLIFERISCGTGYWEPGSGTAPEGGEENIKKYVRILLNDKIQTVAHIACEQSAFAEHGLCEVDAFVESQQFATTNASWDICYNFSREEE
Expression Data
NotationConditionNormalized Raw Expression
AYPD 30°C, concentration: 2x 10^8 cells/ml3241
BYPD 30°C, concentration: 5x10^7 cells/ml1234
CYPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml1303
DYPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml1543
EYPD 37°C, concentration: 5x10^7 cells/ml936
FYP+galactose 30°C, concentration: 2x10^8 cells/ml2493
Gene Disruption Strategy
Mutant construction design Mutant construction design Mutant construction design Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_02681 5' flanking region primer 1ACCAATCCTTTACCCACG
L2CNAG_02681 5' flanking region primer 2TCACTGGCCGTCGTTTTACGATGAAGGCAGCAATGAAC
R1CNAG_02681 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGCGATTCTGATGGTATGCTATGC
R2CNAG_02681 3' flanking region primer 2CGACTCTTCTCCGACAAAC
SOCNAG_02681 diagnostic screening primer, pairing with B79AAGTCAGAGGAATCTCAGCC
POCNAG_02681 Southern blot probe primerCGGAACCTTTCAACAGAACC
STMNAT#150 STM primerACATACACCCCCATCCCCCC
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
* Red marked sequences indicate the extended M13 forward and reversed sequences to overlap PCR with pNAT plasmid.
Phenotype Assays
Growth
Growth phenotype
Mating Data
Mating phenotype
Virulence Factor Production
Capsule
Capsule phenotype
Urease
Urease phenotype
Melanin
Melanin phenotype
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype
Oxidative Stress
Oxidative stress phenotype
Heavy Metal Stress
Heavy metal stress phenotype
Cell Wall Stress
Cell wall stress phenotype
ER Stress
ER stress phenotype
Genotoxic Stress
Genotoxic stress phenotype
Antifungal Drug Susceptibility
Antifungal drug susceptibility
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data