Phosphatase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_01823T1
SpeciesCryptococcus neoformans var. grubii H99
Gene Annotationmyo-inositol-1(or 4)-monophosphatase
Gene SymbolINM1
Phosphatase NameN/A
Phosphatase ClassLipid phosphatase
KEGG Linkout KEGG::CNC03110 
Genome Browser JBrowse link goes here.
Protein Domain / KEGG Annotation
KEGG Result
KO AccessionE-valueScore
K010925.4e-92307.9
Pfam Result
Pfam AccessionE-valueScore
PF00459 Pfam 3.9e-75 251.4
Amino Acid SequenceMTPIELDLDSILQFTIKLALDAGQIIREGQEKRFASESAQEDEKLNSVDLVTEVDKAVEKFIVEKIREAYPSHKFIGEESYEGQQITDEPTWIVDPIDGTTNFVHGFPMVATSIGLAHKGAPVVGVIYNPFLDQLWSAAKGRGAYLNQKRKLPITGSPKPLASLGQALIGVEYGMSRSPPALPRKLAAFEKLTAHTDVGGKMCHSLRSMGSAALNIVLVASGGLDMYWEIGCWPWDVCAGICILEESGGRCFGAKTADLSGEVDAKLMAGRKYVMIRDIRATDKQTSLDQQKKFVKEFYDCVEDFDP
Expression Data
NotationConditionNormalized Raw Expression
AYPD 30°C, concentration: 2x 10^8 cells/ml8498
BYPD 30°C, concentration: 5x10^7 cells/ml11705
CYPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml11016
DYPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml9296
EYPD 37°C, concentration: 5x10^7 cells/ml6664
FYP+galactose 30°C, concentration: 2x10^8 cells/ml7754
Gene Disruption Strategy
Mutant construction design Mutant construction design Mutant construction design Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_01823 5' flanking region primer 1ACTTTAGTCGGTCGGAGATG
L2CNAG_01823 5' flanking region primer 2TCACTGGCCGTCGTTTTACAGTGAGAAGTCTACGGCAC
R1CNAG_01823 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGGAGTTTTACGACTGTGTTGAGG
R2CNAG_01823 3' flanking region primer 2GCATTTCCATCGCACTTCC
SOCNAG_01823 diagnostic screening primer, pairing with B79GCAGAGAGGTATTGAACGAC
POCNAG_01823 Southern blot probe primerACTTACATCAAGGGCGAGC
STMNAT#191 STM primerATATGGATGTTTTTAGCGAG
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
* Red marked sequences indicate the extended M13 forward and reversed sequences to overlap PCR with pNAT plasmid.
Phenotype Assays
Growth
Growth phenotype
Mating Data
Mating phenotype
Virulence Factor Production
Capsule
Capsule phenotype
Urease
Urease phenotype
Melanin
Melanin phenotype
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype
Oxidative Stress
Oxidative stress phenotype
Heavy Metal Stress
Heavy metal stress phenotype
Cell Wall Stress
Cell wall stress phenotype
ER Stress
ER stress phenotype
Genotoxic Stress
Genotoxic stress phenotype
Antifungal Drug Susceptibility
Antifungal drug susceptibility
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data