Phosphatase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_01900T0
SpeciesCryptococcus neoformans var. grubii H99
Gene Annotationhypothetical protein
Gene SymbolNDX1
Phosphatase NameN/A
Phosphatase ClassNucleoside-diphosphate-linked moiety X
KEGG Linkout KEGG::CNK02400 
Genome Browser JBrowse link goes here.
Protein Domain / KEGG Annotation N/A
Amino Acid SequenceMAPKSLLPVVLAADNFPDYPLPPSPPPFPYVPFHLTLLDHQAHLQPLGLLRPDVVRAMQSYNSSLIEAGKGSIWGFVEEGKGDEAGKWQTHVLAVYFKENVASQGKDELGRVMKECLESWKKDGLFPGPLAGWRNELYAIYASPQSSALKNSANKPFGNVAFHLERAACALFGLATFGVHLTAYEGTGQDMKVWVPRRSKTKPTWPGRLDNSVAGGIPAGMTPIDSIIKECDEEASLPEDLVKRYIKNVGVATYFYITADGFLQPEIEYLYDLPLPPQNSEEYVRPAPCDDEVESFALLTIPELIDALHSGDMKPNCGLVYVDFLMRHSFVTPENESHFLEISTRLRRRLGVALPSA
Expression Data
NotationConditionNormalized Raw Expression
AYPD 30°C, concentration: 2x 10^8 cells/ml2916
BYPD 30°C, concentration: 5x10^7 cells/ml3792
CYPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml4915
DYPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml4003
EYPD 37°C, concentration: 5x10^7 cells/ml2925
FYP+galactose 30°C, concentration: 2x10^8 cells/ml2304
Gene Disruption Strategy
Mutant construction design Mutant construction design Mutant construction design Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_01900 5' flanking region primer 1CCATAAAACCACCAGGAGC
L2CNAG_01900 5' flanking region primer 2TCACTGGCCGTCGTTTTACCAACAGGTAAGAGAGATTTGGG
R1CNAG_01900 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGGAATGAATCACACTTCCTGGAG
R2CNAG_01900 3' flanking region primer 2AGACCTCAATGCCGTTCAG
SOCNAG_01900 diagnostic screening primer, pairing with B79GCACCATTTTTTCCTGCTAC
POCNAG_01900 Southern blot probe primerAGTGAGATGGAAAGGCACG
STMNAT#210 STM primerCTAGAGCCCGCCACAACGCT
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
* Red marked sequences indicate the extended M13 forward and reversed sequences to overlap PCR with pNAT plasmid.
Phenotype Assays
Growth
Growth phenotype
Mating Data
Mating phenotype
Virulence Factor Production
Capsule
Capsule phenotype
Urease
Urease phenotype
Melanin
Melanin phenotype
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype
Oxidative Stress
Oxidative stress phenotype
Heavy Metal Stress
Heavy metal stress phenotype
Cell Wall Stress
Cell wall stress phenotype
ER Stress
ER stress phenotype
Genotoxic Stress
Genotoxic stress phenotype
Antifungal Drug Susceptibility
Antifungal drug susceptibility
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data