Phosphatase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_01901T0
SpeciesCryptococcus neoformans var. grubii H99
Gene Annotationhypothetical protein
Gene SymbolNDX2
Phosphatase NameN/A
Phosphatase ClassNucleoside-diphosphate-linked moiety X
KEGG Linkout KEGG::CNK02400 
Genome Browser JBrowse link goes here.
Protein Domain / KEGG Annotation N/A
Amino Acid SequenceMSTHTTSLLPILLQADNLPAYTSPFPTVYPLAKRPIVPFHITFKDFQHGLPPVGLVTMDVVKMLSKEKGIKLFQIAREVIRCSQAGKEGNCKAYPDGNCGKKEQGGGKMKKTFKLTVKFLYFTDEVNARGKKGRTECIARILSDWRAEGKFPQAMKLWMDEMFPIYASPKSSMFNASKPATRELFGNIAFEMERAGVSLFGCQAFGVHLTVRYAYEGQGENMKSVAGGLPAGHTPIQGLIKECGSINDQHRNAEGHKTTRSAGIVTYFEIKDEHILPNAEYTYDLPLPCRDSSDYVLPKPNDDEVDSFELLSVQQLIKALRNGEFKPSSAIVTVDFLIRHGFVSPENEPNYEEVVRRCHRRCGASGPGI
Expression Data
NotationConditionNormalized Raw Expression
AYPD 30°C, concentration: 2x 10^8 cells/ml514
BYPD 30°C, concentration: 5x10^7 cells/ml156
CYPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml170
DYPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml147
EYPD 37°C, concentration: 5x10^7 cells/ml377
FYP+galactose 30°C, concentration: 2x10^8 cells/ml337
Gene Disruption Strategy
Mutant construction design Mutant construction design Mutant construction design Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_01901 5' flanking region primer 1TCGCTTTCTTTCCTCTTCG
L2CNAG_01901 5' flanking region primer 2TCACTGGCCGTCGTTTTACGAAGAGATGTGGTGTGAGTAG
R1CNAG_01901 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGTGAGAAGATGCCACAGGAG
R2CNAG_01901 3' flanking region primer 2GCATCAAAAACGAGCAGAC
SOCNAG_01901 diagnostic screening primer, pairing with B79CCCATCTTTCCCTTTTCTTCTG
POCNAG_01901 Southern blot probe primerGTAGCAGGAAAAAATGGTGC
STMNAT#211 STM primerGCGGTCGCTTTATAGCGATT
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
* Red marked sequences indicate the extended M13 forward and reversed sequences to overlap PCR with pNAT plasmid.
Phenotype Assays
Growth
Growth phenotype
Mating Data
Mating phenotype
Virulence Factor Production
Capsule
Capsule phenotype
Urease
Urease phenotype
Melanin
Melanin phenotype
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype
Oxidative Stress
Oxidative stress phenotype
Heavy Metal Stress
Heavy metal stress phenotype
Cell Wall Stress
Cell wall stress phenotype
ER Stress
ER stress phenotype
Genotoxic Stress
Genotoxic stress phenotype
Antifungal Drug Susceptibility
Antifungal drug susceptibility
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data