Detailed Locus Information for CNAG_02109T0
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | type I inositol-1,4,5-trisphosphate 5-phosphatase 1 | |||||||||
| Gene Symbol | INP5201 | |||||||||
| Phosphatase Name | N/A | |||||||||
| Phosphatase Class | Lipid phosphatase | |||||||||
| KEGG Linkout | KEGG::CNE04280 | |||||||||
| Genome Browser | JBrowse link goes here. | |||||||||
| Protein Domain / KEGG Annotation |
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| Amino Acid Sequence | MASPLHENDEERPQSIAALRSKFESLAIAGVSPAPTDVPSATNGHATVSSIRNGLLSPRPETPVDGQKAKPVPPPKPASRPVSPATTSPAPQPSSLLPPPAPRQAPSRPTTPKPSFQTHHSTSSVTSIVSAASDSHLKPSDTMASPPAVISPAVSPAPTPLRKSAPSVPSKPPSVAVTPSGSDGDEDEPVITSVKALREKFSGQAQASEIALRKPVDVPKASAVSVVKAATVHDSPEPLCAPSATPIPAPIPAPVIQRTLDGKTSPVMLSPASEGEALSDTNDYSSHPTAPLAPPAPPAPISRISSPVPAPAPAPSGPPPINRAHKPPPRTAISPAPIFRPESNVITPNTTSPPIPGNKPVIPSRSSSAPEAAVPPPPPERPQPPQLPVRRPTFSSPDTLEPSTASVISPPALASTPLLHTIHDDTALAPTPAPATAPPPLPDRSRANTINRSESESSATTTGPPPPRLPARHAAIPVSAGSGSTSSNANGSGSTTMNPPPPPAHPASPSKTRINSGGPPPPLLRSATVNRGSSVGSGSGSGGGGGGSPPRRSNTISRAAPFTQEKYSTSATSLGLGEKGVYSDEDDEPEEPGAVTNLSAQAKRMLDEFPDMTEANRRPPVFVPDIRVKECHHVSAFAVYGRYVCTGAHHVRVYDTQLSDHAISVVDLKETGLESRGKDPKVTAMCFRPGATESEEGRYLWCGTKDGHLWELDISTGEVTSTKAFVHTSSISYIWRHRKNIISLDEGGKLLVFDVGDIEGKPPTMARQLRIGDKFGFAKLICGKLWTSSGPLTRSTTSSATSKGPTVRIYDPCAPGTMPPPKTIFATEWAGAVTSATYMPLHHDTIFLGHEGGFVSVWDGKELVCKQVLKISSTDVLALEGVGEYLWTGNRKGQIHVFDIKEKPWLATNIWIGHPDNPVQSLVVDPYSIQSAGRYTCWSFARDALRAWDGLLSVDWIDKQLTARQSSFCTFRPVNVLICTWNIDSAKPTDLNGSVANAHFLEDVLRSVDSPDIIVFGFQEVIPLTDKKYTAKTLLFGNKSKDGGAAADRVSHAYRHWLEKLQSAVQMASPSNCPYIKIHSESLVGLFTCIFVKQSEKISLRDLDITTVKRGIGGIYGNKGAIVSRLVMDDTSICFINVHLAAGQSQKASRNADLAGILEDKAIFPPADELPFVHGGCGTGILDHEMVFLNGDLNYRIDQRRENVISSIANGELAYLLEHDQLRKEMRTNHAFRLRNFEEAPITFAPTYKYDPGTHDYDSSEKRRIPAWCDRILYKKSPRVQALNYQRYEPTVSDHRPVSAGYTIILKAIDSLKMMDVRREATGEWAKREKELLEKMQEVFDGIE | |||||||||
Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 5843 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 5353 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 5690 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 4356 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 5017 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 5273 |
Gene Disruption Strategy
| Primer Name | Description | Primer Sequence |
|---|---|---|
| L1 | CNAG_02109 5' flanking region primer 1 | CGTTTCCATTTGGGGTCAG |
| L2 | CNAG_02109 5' flanking region primer 2 | TCACTGGCCGTCGTTTTACTGCGAGGCTCTCAAACTTG |
| R1 | CNAG_02109 3' flanking region primer 1 | CATGGTCATAGCTGTTTCCTGCGAAGCGAGAAAAGGAGTTG |
| R2 | CNAG_02109 3' flanking region primer 2 | TTCTTCCTCACCTGGATACCGC |
| SO | CNAG_02109 diagnostic screening primer, pairing with B79 | AGTCTTGATGGCTTCTTCAC |
| PO | CNAG_02109 Southern blot probe primer | GCTGTTTAGAGTGAGTAGAGG |
| STM | NAT#122 STM primer | ACAGCTCCAAACCTCGCTAAACAG |
| STM common | STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
* Red marked sequences indicate the extended M13 forward and reversed sequences to overlap PCR with pNAT plasmid.
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule  Urease  Melanin  | |
| Stress Response and Adaptation | |
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Osmotic Stress  Oxidative Stress  Heavy Metal Stress  Cell Wall Stress  ER Stress  Genotoxic Stress  | |
| Antifungal Drug Susceptibility | |
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| Virulence Data by the Insect Host Model (Galleria mellonella) | |
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| Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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