Phosphatase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_04354T0
SpeciesCryptococcus neoformans var. grubii H99
Gene Annotationexopolyphosphatase
Gene SymbolXPP1
Phosphatase NameN/A
Phosphatase ClassExopolyphosphatase
KEGG Linkout KEGG::CNI01960 
Genome Browser JBrowse link goes here.
Protein Domain / KEGG Annotation
KEGG Result
KO AccessionE-valueScore
K015146e-101337.5
Pfam Result
Pfam AccessionE-valueScore
PF02833 Pfam 1.2e-20 69.6
Amino Acid SequenceMRLPRHSSLICLLLAFITIPPVIFILLFLDYFDSSMRVCLPFSLARLSTTAHNTLSCQARPVSSIIMNDATIQSGNDNATVPEGRLAGFLSSQRDLFLQDLKEGKGKGWTVVMGNQAGDLDSLASSVAFSQLSATLLASRVVPLILSPPKSMGLRPENLLALRNTSIPLTSLLHASQLPVSTTELASQGITFTLVDHNKLLPQFGQGKVNAIIDHHEDENAHTDAPIREITIPTGSCASLVVKHFRPQWEASISRGSPVPPELATLLLSAIVIDTGGLKPGGKATPVDYEAASFLYSISTIAQGQSGSFSITNEVGLPPSLKLLSDTLHDAKSDVANLTTYELLMRDYKEYEWPTQSRNFPTLKVGLSTVPLGLKTWIAKEQDGWETLMSSTEQYMQEGTLDIEGILTTYRSEKKGKHKRQLVLIVRPGGVIRDPQEARSVFERLVGGLEASEVLQLEEWKKEEKVKRQGDERLVKVWVQGNAKATRKQIAPLLKNLMSELQ
Expression Data
NotationConditionNormalized Raw Expression
AYPD 30°C, concentration: 2x 10^8 cells/ml7759
BYPD 30°C, concentration: 5x10^7 cells/ml6786
CYPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml7476
DYPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml4285
EYPD 37°C, concentration: 5x10^7 cells/ml3026
FYP+galactose 30°C, concentration: 2x10^8 cells/ml5477
Gene Disruption Strategy
Mutant construction design Mutant construction design Mutant construction design Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_04354 5' flanking region primer 1GGCAATGGGGACAGTAAAC
L2CNAG_04354 5' flanking region primer 2TCACTGGCCGTCGTTTTACACAGGAGAGTGTGTTGTGG
R1CNAG_04354 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGGTCTGTTCAACGAGATGGC
R2CNAG_04354 3' flanking region primer 2TAACCAACCGTCTGACCTC
SOCNAG_04354 diagnostic screening primer, pairing with B79TGAAAGCACTCTCCTGAGG
POCNAG_04354 Southern blot probe primerAGGAGGCAAGAGAGTCAAG
STMNAT#234 STM primerCCAGCCAGCACTCCGATATA
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
* Red marked sequences indicate the extended M13 forward and reversed sequences to overlap PCR with pNAT plasmid.
Phenotype Assays
Growth
Growth phenotype
Mating Data
Mating phenotype
Virulence Factor Production
Capsule
Capsule phenotype
Urease
Urease phenotype
Melanin
Melanin phenotype
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype
Oxidative Stress
Oxidative stress phenotype
Heavy Metal Stress
Heavy metal stress phenotype
Cell Wall Stress
Cell wall stress phenotype
ER Stress
ER stress phenotype
Genotoxic Stress
Genotoxic stress phenotype
Antifungal Drug Susceptibility
Antifungal drug susceptibility
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data