Phosphatase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_02487T0
SpeciesCryptococcus neoformans var. grubii H99
Gene Annotation3-hydroxy acyl-CoA dehydratase
Gene SymbolPHS1
Phosphatase NameN/A
Phosphatase ClassProtein tyrosine phosphatase
KEGG Linkout KEGG::CNE00730 
Genome Browser JBrowse link goes here.
Protein Domain / KEGG Annotation
KEGG Result
KO AccessionE-valueScore
K107039.4e-65217.2
Pfam Result
Pfam AccessionE-valueScore
PF04387 Pfam 5.2e-50 166.7
Amino Acid SequenceMFPSDTKASLHSAFQSVLDSASPSILARLSLQLAAMPHVAAPSRQALEQERLHRHAQQPGSSLSPAPRLSPLRLYLLGYNILSALLWGHLLVLTLSFLLAPSRPPWHQLADRLSGSYDYHNLGWCTKWTQTLAVLEVVHAALGWVRSPLGTVASQVASRLWTVWGVVEAAPEITHGHPLFTTMLLAWSLTEVIRYSFYALSLLSVSAPFLNYLRYTTFIPLYPLGASSEAFLSFATLPALAPVVSRAVTNVMAQAPREIMKTKVGREVLWWSAKHGGGTAGAQKEWGWIEIVRAGLFLLWWPALYVLYTYMLKQRRKVLGKGKTVGGVSKAQ
Expression Data
NotationConditionNormalized Raw Expression
AYPD 30°C, concentration: 2x 10^8 cells/ml1781
BYPD 30°C, concentration: 5x10^7 cells/ml1669
CYPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml1753
DYPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml1658
EYPD 37°C, concentration: 5x10^7 cells/ml1278
FYP+galactose 30°C, concentration: 2x10^8 cells/ml1777
Gene Disruption Strategy
Mutant construction design Mutant construction design Mutant construction design Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_02487 5' flanking region primer 1GTGGGATGGGAAATGATG
L2CNAG_02487 5' flanking region primer 2CTGGCCGTCGTTTTACAGTGACGCTTTTGTGTCG
R1CNAG_02487 3' flanking region primer 1GTCATAGCTGTTTCCTGTAAAGGCAAGACCGTAGGC
R2CNAG_02487 3' flanking region primer 2CCTTCGTCTTCTTCGTCTC
SOCNAG_02487 diagnostic screening primer, pairing with B79ACACTGAAGAGACTCCCGAG
POCNAG_02487 Southern blot probe primerTGAGTAGCGGATGACTTCG
STMNAT#225 STM primerCCATAGAACTAGCTAAAGCA
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
* Red marked sequences indicate the extended M13 forward and reversed sequences to overlap PCR with pNAT plasmid.
Phenotype Assays
Growth
Growth phenotype
Mating Data
Mating phenotype
Virulence Factor Production
Capsule
Capsule phenotype
Urease
Urease phenotype
Melanin
Melanin phenotype
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype
Oxidative Stress
Oxidative stress phenotype
Heavy Metal Stress
Heavy metal stress phenotype
Cell Wall Stress
Cell wall stress phenotype
ER Stress
ER stress phenotype
Genotoxic Stress
Genotoxic stress phenotype
Antifungal Drug Susceptibility
Antifungal drug susceptibility
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data