Detailed Locus Information for CNAG_02545T0
| Species | Cryptococcus neoformans var. grubii H99 | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | inorganic pyrophosphatase | ||||||||||||||||||
| Gene Symbol | IPP1 | ||||||||||||||||||
| Phosphatase Name | N/A | ||||||||||||||||||
| Phosphatase Class | Pyrophosphatase | ||||||||||||||||||
| KEGG Linkout | KEGG::CNE00210 | ||||||||||||||||||
| Genome Browser | JBrowse link goes here. | ||||||||||||||||||
| Protein Domain / KEGG Annotation |
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| Amino Acid Sequence | MNTTAVRRLSSTLSHLNRNIPKMAYQTRIIGAANTLEHRVYVEQEGKVVSPFHDIPLFADESKTILNMVVEVPRWTNAKMEISKEETFNPIKQDIKKGKLRYVRNCFPHHGYIWNYGAFPQTWEDPNVKHAETGANGDNDPLDVCEIGEAVAYTGQVKQVKVLGIMALLDEGETDWKVLVVDVNDPLAPRLNDIEDVERHLPGLIRATNEWFRIYKIPDGKPENVFAFSGEAKSKKYAVEIIHECHEAWRKLVHGETAASTDAYNLAITNTTVKGSKGFVSTNDAAYASIPADSRKPAAPIDPSIDKSFFISSASA | ||||||||||||||||||
Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 14277 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 32374 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 30871 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 36595 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 26677 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 14540 |
Gene Disruption Strategy
| Primer Name | Description | Primer Sequence |
|---|---|---|
| L1 | CNAG_02545 5' flanking region primer 1 | TGCCTACTTTTCCGCTGTC |
| L2 | CNAG_02545 5' flanking region primer 2 | TCACTGGCCGTCGTTTTACGCGAGAGAGTAGAGGATAATCG |
| R1 | CNAG_02545 3' flanking region primer 1 | CATGGTCATAGCTGTTTCCTGTTCTTCATCTCCTCCGCCTCTG |
| R2 | CNAG_02545 3' flanking region primer 2 | CGCCTGATTTGTGATTTCTCC |
| SO | CNAG_02545 diagnostic screening primer, pairing with B79 | TGAGGGCGGAGACAAAGTTG |
| PO | CNAG_02545 Southern blot probe primer | GCTCTGTCATCTTATCGCAAC |
| STM | NAT#210 STM primer | CTAGAGCCCGCCACAACGCT |
| STM common | STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
* Red marked sequences indicate the extended M13 forward and reversed sequences to overlap PCR with pNAT plasmid.