Detailed Locus Information for CNAG_04453T0
| Species | Cryptococcus neoformans var. grubii H99 | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | inositolphosphorylceramide synthase | ||||||||||||||||||
| Gene Symbol | IPC1 | ||||||||||||||||||
| Phosphatase Name | N/A | ||||||||||||||||||
| Phosphatase Class | Lipid phosphatase | ||||||||||||||||||
| KEGG Linkout | KEGG::CNI01040 | ||||||||||||||||||
| Genome Browser | JBrowse link goes here. | ||||||||||||||||||
| Protein Domain / KEGG Annotation |
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| Amino Acid Sequence | MSAIRALTSPVTACFSTSLSPHAAFHRFLHALSASIRRLDLSRDPRKTLDRLRQHRFTLANTLPRAFMLLCASYSLYIMTTPPFPLKLGIPIAYIAAVIFPITSQFVWPATPIFAWLITFFSARFIPSGRRPEIHVALLPALESVLYGANISDLQTRYTNAFLDVVAWLPYGVLHFTLPFVVAVILWSLGPRGAVQFWGLAFGWMNLLGVVCQLLFPAAAPWYEIIHGLTPADYSMAGSPGGLMRIDRVFHSSGYTNAFGSAPLVFGAFPSLHSGCAVMEALFLSHFFPSLKGLYWGYVGVLWWATMYLSHHYLIDLVGGACLSVLVFYLCMPEGFKDVDQIQWEAVEGDGYEMIGGPRTGTGPEIDLDEEIRKLEEQGEALFEQVIGDEESRIETRGEGNSGAGGNLSGNESGDSADSGRGKGKGKGKGKQTAKKPKAKEQRSVSWGETKVMGEGAQVASENSS | ||||||||||||||||||
Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 1233 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 1445 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 1639 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 1506 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 1752 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 1012 |
Gene Disruption Strategy
| Primer Name | Description | Primer Sequence |
|---|---|---|
| L1 | CNAG_04453 5' flanking region primer 1 | CCTGGGCTATGGAATGTATC |
| L2 | CNAG_04453 5' flanking region primer 2 | TCACTGGCCGTCGTTTTACAGAGAGAGGTGGAGAAGCAG |
| R1 | CNAG_04453 3' flanking region primer 1 | CATGGTCATAGCTGTTTCCTGGGTGGCTTCAGAGAATAGC |
| R2 | CNAG_04453 3' flanking region primer 2 | GTCTTCCTCGTCTTACATCC |
| SO | CNAG_04453 diagnostic screening primer, pairing with B79 | TGACCCCTTGTCTCGTTAC |
| PO | CNAG_04453 Southern blot probe primer | AAAGTGTTGGCAAGGGTG |
| STM | NAT#213 STM primer | CTGGGGATTTTGATGTGTCTATGT |
| STM common | STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
* Red marked sequences indicate the extended M13 forward and reversed sequences to overlap PCR with pNAT plasmid.