Phosphatase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_03052T0
SpeciesCryptococcus neoformans var. grubii H99
Gene AnnotationPP2Cc protein phosphatase
Gene SymbolPTC2
Phosphatase NameN/A
Phosphatase ClassMetallo-dependent phosphatase
KEGG Linkout KEGG::CNC00360 
Genome Browser JBrowse link goes here.
Protein Domain / KEGG Annotation
KEGG Result
KO AccessionE-valueScore
K148036.2e-128426.2
Pfam Result
Pfam AccessionE-valueScore
PF00481 Pfam 1.4e-70 229.3
Amino Acid SequenceMGQTLSEPMTEKHTTDVVRGKQFWVGLSDMQGWRISMEDAHSVHLYLPPSSDDSKPYSPGSDIPAQPEGSTVTNNNEPEVANAMFGVFDGHGGQTVAKFAGTTLHSRLSALDTYKSGDYTAALTQAFIKTDEDLRADPSFLNDPSGCTAVVGLITTDGRIIVANSGDSRSVLGYQGQAKALSNDHKPTNEEETARITAAGGFVEFGRVNGNLALSRAMGDFEFKQNFSLAPEKQIVTVVPEIITHTLDGEEEFLVLACDGIWDCLTSQQVIDFTRRAIANGDPLGKICENMMVKCLAKDSSTGGIGCDNMTVVIVALLNGRTPEEWQAWVKERVEQKVGHDTPESIPDVFPQSSGPSNSGFGGSGFRVAGGAGGLANIASILGASGITFRPAYDSDDDDDGIQLIGDDPASPDKLSSPGLIDNEAVEGETKPKDVSGQFEKEEEETLFGGEGKKGSVRLLDEDGDSSMDSDDSDSTTTGNDPAPQPIQSSSHQAIHSPIPPNFSSIGSPTVPTPQELQGLQASNRSEPQEDAYSDAVKVEGLMDKSESPLNL
Expression Data
NotationConditionNormalized Raw Expression
AYPD 30°C, concentration: 2x 10^8 cells/ml11974
BYPD 30°C, concentration: 5x10^7 cells/ml13131
CYPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml12797
DYPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml12453
EYPD 37°C, concentration: 5x10^7 cells/ml9120
FYP+galactose 30°C, concentration: 2x10^8 cells/ml11327
Gene Disruption Strategy
Mutant construction design Mutant construction design Mutant construction design Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_03052 5' flanking region primer 1AGAGTGGAAGAAGGAAGACG
L2CNAG_03052 5' flanking region primer 2TCACTGGCCGTCGTTTTACTTCCGTCACTGTTGTTCCAA
R1CNAG_03052 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGCAAGAACTTCAGGGACTGC
R2CNAG_03052 3' flanking region primer 2ATGAGGAGTAGCCTGTTGC
SOCNAG_03052 diagnostic screening primer, pairing with B79GCGGAAGGTAAAAAAGTGTG
PO1CNAG_03052 Southern blot probe primer 1GCAGGGTCAAACACTCTCTG
PO2CNAG_03052 Southern blot probe primer 2GCAAGGTTACCGTTCACTC
STMNAT#224 STM primerAACCTTTAAATGGGTAGAG
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
* Red marked sequences indicate the extended M13 forward and reversed sequences to overlap PCR with pNAT plasmid.
Phenotype Assays
Growth
Growth phenotype
Mating Data
Mating phenotype
Virulence Factor Production
Capsule
Capsule phenotype
Urease
Urease phenotype
Melanin
Melanin phenotype
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype
Oxidative Stress
Oxidative stress phenotype
Heavy Metal Stress
Heavy metal stress phenotype
Cell Wall Stress
Cell wall stress phenotype
ER Stress
ER stress phenotype
Genotoxic Stress
Genotoxic stress phenotype
Antifungal Drug Susceptibility
Antifungal drug susceptibility
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data