Phosphatase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_03222T0
SpeciesCryptococcus neoformans var. grubii H99
Gene AnnotationRNA lariat debranching enzyme
Gene SymbolDBR1
Phosphatase NameN/A
Phosphatase ClassPhosphoprotein phosphatase
KEGG Linkout KEGG::CNG03510 
Genome Browser JBrowse link goes here.
Protein Domain / KEGG Annotation
KEGG Result
KO AccessionE-valueScore
K183282.6e-164546.3
Pfam Result
Pfam AccessionE-valueScore
PF05011 Pfam 1.8e-33 113.7
Amino Acid SequenceMRIAIQGCSHGSLAQIYDVVNYYSSQTKNPIDLLLLCGDFQALRSKHDYASLAVPAKFKQLGSFHQYYSGERVAPVLTIVIGGNHEASNYMWELYHGGWLAPSIYYLGAAGSVYVNGVRIVGASGIYKGFDYRKGHFEKVPYNDKELRSVYHIREYDVEKLMHLTPSPSTIFLSHDWPTTIAHHGNKNALLKRKPFFRDEIEKNTLGSPPLLRLMNHFQPSYWFSAHLHVKFAALYEHQAPNHGPDVDGGAPLPLLATSAVIAQAGGNPDEIQIDEEMDAGNPDEIIVEDEGEEVIIRPRQVNPDEIAMDDDEFDDPAPAVPQPLPATTNSAFNPEEIIISDQEFDAPTTVSQPLQPLPPTKTNASNPEEIAISDDEFDDPAPLAQSLTTIDESTDLIAQSRSNPSHPPVAGTIAPPTSDSIASRVMQEARQEQQKWELHGGKGMEGVTKFLALDKCGPGKDHMQFLEIPDPSPPPIPGPPRLTYDPEWLAISRAFHPYLSTSYQPIPLPSSDILEQMVKDEVARIKEEGLLVPTVPQDGAVEGQEGLVWEKGKVDVGRVQRFWWTAPPEGHPGGNDTAWYTNPQTEAFCGMLGVQNKINPPVNR
Expression Data
NotationConditionNormalized Raw Expression
AYPD 30°C, concentration: 2x 10^8 cells/ml865
BYPD 30°C, concentration: 5x10^7 cells/ml1154
CYPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml804
DYPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml1300
EYPD 37°C, concentration: 5x10^7 cells/ml1430
FYP+galactose 30°C, concentration: 2x10^8 cells/ml855
Gene Disruption Strategy
Mutant construction design Mutant construction design Mutant construction design Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_03222 5' flanking region primer 1TTCCAATCCAACAGTCGC
L2CNAG_03222 5' flanking region primer 2TCACTGGCCGTCGTTTTACTCCGAAATGCCTGTTGAGG
R1CNAG_03222 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGTTACTCCCACTCGCTAAGC
R2CNAG_03222 3' flanking region primer 2TTTTGGTAGGTGGGAGAGG
SOCNAG_03222 diagnostic screening primer, pairing with B79AGAAATAGAAAGGCTGGCG
POCNAG_03222 Southern blot probe primerTACTGACCCTCATACTGCG
STMNAT#282 STM primerTCTCTATAGCAAAACCAATC
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
* Red marked sequences indicate the extended M13 forward and reversed sequences to overlap PCR with pNAT plasmid.
Phenotype Assays
Growth
Growth phenotype
Mating Data
Mating phenotype
Virulence Factor Production
Capsule
Capsule phenotype
Urease
Urease phenotype
Melanin
Melanin phenotype
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype
Oxidative Stress
Oxidative stress phenotype
Heavy Metal Stress
Heavy metal stress phenotype
Cell Wall Stress
Cell wall stress phenotype
ER Stress
ER stress phenotype
Genotoxic Stress
Genotoxic stress phenotype
Antifungal Drug Susceptibility
Antifungal drug susceptibility
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data