Phosphatase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_03296T0
SpeciesCryptococcus neoformans var. grubii H99
Gene Annotationtyrosine specific protein phosphatase family protein
Gene SymbolSIW14
Phosphatase NameN/A
Phosphatase ClassProtein tyrosine phosphatase
KEGG Linkout KEGG::CNG02810 
Genome Browser JBrowse link goes here.
Protein Domain / KEGG Annotation
KEGG Result
KO AccessionE-valueScore
K180454.1e-92307.8
Pfam Result
Pfam AccessionE-valueScore
PF03162 Pfam 2.5e-56 188.2
Amino Acid SequenceMMSSSPTMSSTPPQVPSFLANILLSHLSPLDPSSTSPTTFEHHRSPTTQNPSHPQALQTAEPPPNPLYLPPPALPKVEEDLVPPENFALVSSGVYRCGFPKKRNFKFMETLRLKTVLTLVLEEYPKANLEWCQSQDIQFMQFGIPGNKEPFDNIPEDVICAALVAILDRRNHPILIHCNKGKHRTGCLIGCIRRLQAWSLTSIFDEYRRFSAPKSRAVDQQFIDLFDIMPVWEAVCRPKGGGLGNLPDWGMLVLPKGVVEVGRDGKEKKRVERDILHMRGL
Expression Data
NotationConditionNormalized Raw Expression
AYPD 30°C, concentration: 2x 10^8 cells/ml741
BYPD 30°C, concentration: 5x10^7 cells/ml1108
CYPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml1218
DYPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml1141
EYPD 37°C, concentration: 5x10^7 cells/ml1247
FYP+galactose 30°C, concentration: 2x10^8 cells/ml729
Gene Disruption Strategy
Mutant construction design Mutant construction design Mutant construction design Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_03296 5' flanking region primer 1CTCATTCAGGATTTACCACG
L2CNAG_03296 5' flanking region primer 2TCACTGGCCGTCGTTTTACAAAGTTGTTGGCGAGGTC
R1CNAG_03296 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGTCATCGGGAGTTTGTTCAG
R2CNAG_03296 3' flanking region primer 2CAACTACCACTCACAACTCTTG
SOCNAG_03296 diagnostic screening primer, pairing with B79GTTTGTCCAGTTTGGCAAG
POCNAG_03296 Southern blot probe primerAGTATGGGATGATTCCGC
STMNAT#225 STM primerCCATAGAACTAGCTAAAGCA
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
* Red marked sequences indicate the extended M13 forward and reversed sequences to overlap PCR with pNAT plasmid.
Phenotype Assays
Growth
Growth phenotype
Mating Data
Mating phenotype
Virulence Factor Production
Capsule
Capsule phenotype
Urease
Urease phenotype
Melanin
Melanin phenotype
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype
Oxidative Stress
Oxidative stress phenotype
Heavy Metal Stress
Heavy metal stress phenotype
Cell Wall Stress
Cell wall stress phenotype
ER Stress
ER stress phenotype
Genotoxic Stress
Genotoxic stress phenotype
Antifungal Drug Susceptibility
Antifungal drug susceptibility
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data