Phosphatase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_03491T0
SpeciesCryptococcus neoformans var. grubii H99
Gene Annotationcleavage and polyadenylation specificity factor subunit 5
Gene SymbolNDX3
Phosphatase NameN/A
Phosphatase ClassNucleoside-diphosphate-linked moiety X
KEGG Linkout KEGG::CNG01010 
Genome Browser JBrowse link goes here.
Protein Domain / KEGG Annotation
KEGG Result
KO AccessionE-valueScore
K143972.9e-85284.7
Pfam Result
Pfam AccessionE-valueScore
PF13869 Pfam 2.5e-66 221.2
Amino Acid SequenceMVDNTHNTIEAFPLRNYLFIEREGQPEEDNSVTNRLKRLEDQYTESGTRRSVEAIMVVTVHGFPHVLVLQVANAFYKLPGGYLDPSESDAEGLITRLNEQLGVPVTTFKGKGEDDLPHTVWLAPEEGRDWEVRDCLSVWYRPHFDTFIYPYAPAHVSYPKECKKLYLVNLPPNKTFAVPANMKLHAIPVFEFYDNAARYGPQFAGIPYILSRFVMSQPEVCEEDEHKDYKAVVPNNL
Expression Data
NotationConditionNormalized Raw Expression
AYPD 30°C, concentration: 2x 10^8 cells/ml456
BYPD 30°C, concentration: 5x10^7 cells/ml551
CYPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml352
DYPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml450
EYPD 37°C, concentration: 5x10^7 cells/ml315
FYP+galactose 30°C, concentration: 2x10^8 cells/ml443
Gene Disruption Strategy
Mutant construction design Mutant construction design Mutant construction design Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_03491 5' flanking region primer 1CCGAATCTTATCGTTCTCCTAC
L2CNAG_03491 5' flanking region primer 2TCACTGGCCGTCGTTTTACCCTATCGCAGGCTATCAGTG
R1CNAG_03491 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGCGAGCACAAAGACTACAAAGC
R2CNAG_03491 3' flanking region primer 2GTTCAGACATTCCAAGGCG
SOCNAG_03491 diagnostic screening primer, pairing with B79TCCTCCTATGTCAGGGTTCG
POCNAG_03491 Southern blot probe primerGTTACTGAGTTGTCTTCCTCTG
STMNAT#201 STM primerCACCCTCTATCTCGAGAAAGCTCC
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
* Red marked sequences indicate the extended M13 forward and reversed sequences to overlap PCR with pNAT plasmid.
Phenotype Assays
Growth
Growth phenotype
Mating Data
Mating phenotype
Virulence Factor Production
Capsule
Capsule phenotype
Urease
Urease phenotype
Melanin
Melanin phenotype
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype
Oxidative Stress
Oxidative stress phenotype
Heavy Metal Stress
Heavy metal stress phenotype
Cell Wall Stress
Cell wall stress phenotype
ER Stress
ER stress phenotype
Genotoxic Stress
Genotoxic stress phenotype
Antifungal Drug Susceptibility
Antifungal drug susceptibility
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data