Phosphatase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_03765T0
SpeciesCryptococcus neoformans var. grubii H99
Gene Annotationtrehalose-phosphatase
Gene SymbolTPS2
Phosphatase NameN/A
Phosphatase ClassHaloacid dehalogenase
KEGG Linkout KEGG::CNB02610 
Genome Browser JBrowse link goes here.
Protein Domain / KEGG Annotation
KEGG Result
KO AccessionE-valueScore
K160551.6e-287797.3
K160551.6e-287156
Pfam Result
Pfam AccessionE-valueScore
PF00982 Pfam 1.2e-141 365.8
PF00982 Pfam 1.2e-141 102.3
PF02358 Pfam 6.7e-72 238
Amino Acid SequenceMDSMHPDPTPEVPPSLPDLKAQVARLEASHKEKGLPLSGRIIHVMHHLPVEIVRIVPADALESAGTGFLSPPMTPEFKPEDAETTVESADAKWRIHARTAHPALVSGIKSLSETHDQLLVAWTGEVLIQPDNTQSPQQPSQATFSSIASNLLAPFSAGDATPQPQPPPQQGSLMVFGGEFNEADQKEIASELDRFAEAEAKFDPTDRLKYIPVFLPPDVSKGHYEGFCKKTLWPLFHYLLWLDSTATVPSPDPSWLAYQKTNQMFAQRVAQIYKPGDLIICHDYHLLLAPKMIRESLGQVFHPNAGWGTAHPSPSLHHAQRKGFDWESNQQTPTPEKAKGEKIGAFMNHVGTALGNHLGTMEHGLQNEIMIGMFMHTPWPSSEIFRCLPKRREILDGMLGANLVSFQTYSYSRHFVSTCIRVCGYESTPGGVDANGQVTAVGYCPIGIDIKRVVHDRDQPGVLPKMQALRDLYKDKKIIVGREKLDVAKGVYNKLQAFEKFLQVYPEWRGKVVLIQVTTPALSESPKLERMTAELVSHINGTYGSLDFTPVHHYHQALEKDEYFGLLSCADLALITSLRDGMNTTSMEFILCQDKTSKSPLVLSEFMGTAPSFASALQINPHDLLGVAQAINKGLTMRKEEKAERHANLLEGVLAHTSHTWAATILKQLLENVGGEHTAHHTPALDTAQFTDAYKKANKRLLLFDYDGTLTPIVKVPSHAVPTERTRNAIAALCKDPKNVVYLISGRDGDFLEEHWGHLDRLGLSAEHGSFVKQPGEEDFINMTEALDMSWMSEVEEIFKYYTERTTGSTIEVKKASITWHYRNSDPDFGEFQCKQALDLLESSLAPRRPIEVLVGKKNLEVRPLAVNKGEIVRRLMYENPDVDLIFCAGDDKTDEDMFRALRTIFPPGGVVDNNPVVMKPPVAVTSALEPEEVAELPDVELTIRSKGVFATTVGPPAKRTLAGWHVTCPEEVVEAFESLLEEIQVV
Expression Data
NotationConditionNormalized Raw Expression
AYPD 30°C, concentration: 2x 10^8 cells/ml82654
BYPD 30°C, concentration: 5x10^7 cells/ml66956
CYPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml71681
DYPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml35574
EYPD 37°C, concentration: 5x10^7 cells/ml33850
FYP+galactose 30°C, concentration: 2x10^8 cells/ml59681
Gene Disruption Strategy
Mutant construction design Mutant construction design Mutant construction design Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_03765 5' flanking region primer 1GGCTGGTGTGAAAGAAGTTATCTC
L2CNAG_03765 5' flanking region primer 2TCACTGGCCGTCGTTTTACAAGCCCTTTCTCCTTGTGG
R1CNAG_03765 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGGGAGGTTGTTGAGGCTTTTGAATC
R2CNAG_03765 3' flanking region primer 2AATCTCACAGACGACGACGG
SOCNAG_03765 diagnostic screening primer, pairing with B79GGATTGAGGGTGGATTGAAC
POCNAG_03765 Southern blot probe primerATCTTCGGGCTTGAACTCG
STMNAT#232 STM primerCTTTAAAGGTGGTTTGTG
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
* Red marked sequences indicate the extended M13 forward and reversed sequences to overlap PCR with pNAT plasmid.
Phenotype Assays
Growth
Growth phenotype
Mating Data
Mating phenotype
Virulence Factor Production
Capsule
Capsule phenotype
Urease
Urease phenotype
Melanin
Melanin phenotype
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype
Oxidative Stress
Oxidative stress phenotype
Heavy Metal Stress
Heavy metal stress phenotype
Cell Wall Stress
Cell wall stress phenotype
ER Stress
ER stress phenotype
Genotoxic Stress
Genotoxic stress phenotype
Antifungal Drug Susceptibility
Antifungal drug susceptibility
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data