Detailed Locus Information for CNAG_04782T1
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | hypothetical protein | |||||||||
| Gene Symbol | PPU1 | |||||||||
| Phosphatase Name | N/A | |||||||||
| Phosphatase Class | Phosphoprotein phosphatase | |||||||||
| KEGG Linkout | KEGG::CNJ02090 | |||||||||
| Genome Browser | JBrowse link goes here. | |||||||||
| Protein Domain / KEGG Annotation |
|
|||||||||
| Amino Acid Sequence | MTSQTEEGDNEEVGNETGNSNGTDTTFIPSIPLDNWDPLAMHSTGLTEITVKSCYFPPEIFPSFCAPETTPELDKTKGKWVIVEKDLNVRSGLWYLNLYYRRTRRLDAKLITDIKVLSHPPENEEQLYEDGWVLAPGDLHNGVWPKQTEMRLWYKLGHQGWDDWRKEGKRREDDHDSAVDGEAESSETESSGDQDQGTSSSGEVQEIDEGSKTATSYGGFINEIDVLYGDDDPFFGFERVQGGPVLKEEKGKWETVDITVRRGNPRPPRATVPTFHSDGTLKIMQIADLHYSVGTGECRDTDLEGCVGDANTAAWIAEALDAENPDLVVFSGDQLNGQRTSYDARSVLAKFAKPVIERKIPWCAVFGNHDSEIYGDRDYQMKTLENMPYSLSRAGPKSVDGVGNYYIKLHSSDASDMHIFTLYFLDSHAYQKRTLPWAKPDYDYLKTSQIEWYRNVSSSIKPIERPFKPDGAEDLSGIWSRRSQASRLPRDGSQTLAKPNAMMWFHIPLPEAYNDPDQSLMGELNVGVQMDIVGSSKHNSGFFYNAIKTTYDSEETEGYFNKKTAEVKVLNHGHCHNTDRCRRVDGIWMCFGGGSSFSGYGQLGFDRRVRVYRISEYGEKVETYKRLTSGEIIDEQVLVGDGAAEGVGQDQI | |||||||||
Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 9829 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 4753 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 6582 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 4102 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 5006 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 9740 |
Gene Disruption Strategy
| Primer Name | Description | Primer Sequence |
|---|---|---|
| L1 | CNAG_04782 5' flanking region primer 1 | TGACATAAACGGCAACTCC |
| L2 | CNAG_04782 5' flanking region primer 2 | TCACTGGCCGTCGTTTTACATGCTGTGGTGAGTAAGGG |
| R1 | CNAG_04782 3' flanking region primer 1 | CATGGTCATAGCTGTTTCCTGCATACAAGAGGTTGACAAGTGG |
| R2 | CNAG_04782 3' flanking region primer 2 | TGAGCAGTTTTTTGCCTCG |
| SO | CNAG_04782 diagnostic screening primer, pairing with B79 | ATCCTTTGTCGTGCGTTC |
| PO | CNAG_04782 Southern blot probe primer | AGAGGAAGAAGAAGAGGAGG |
| STM | NAT#212 STM primer | AGAGCGATCGCGTTATAGAT |
| STM common | STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
* Red marked sequences indicate the extended M13 forward and reversed sequences to overlap PCR with pNAT plasmid.
Phenotype Assays
| Growth | |
|---|---|
 | |
| Mating Data | |
 | |
| Virulence Factor Production | |
|
Capsule  Urease  Melanin  | |
| Stress Response and Adaptation | |
|
Osmotic Stress  Oxidative Stress  Heavy Metal Stress  Cell Wall Stress  ER Stress  Genotoxic Stress  | |
| Antifungal Drug Susceptibility | |
 | |
| Virulence Data by the Insect Host Model (Galleria mellonella) | |
 | |
| Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
 |