Detailed Locus Information for CNAG_03957T0
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | 4-nitrophenyl phosphatase | |||||||||
| Gene Symbol | Unknown | |||||||||
| Phosphatase Name | N/A | |||||||||
| Phosphatase Class | Haloacid dehalogenase | |||||||||
| KEGG Linkout | KEGG::CNB04450 | |||||||||
| Genome Browser | JBrowse link goes here. | |||||||||
| Protein Domain / KEGG Annotation |
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| Amino Acid Sequence | MAPPFLKSVEEYEELVDSVDTFLLDCDGVLYHGKQVVEGVRTVLNMLRKKGKKIIFVTNNATKSRRKLKETFDQLGLNASIDECFGSAYASAVYISQVLNFPKDKKVYVFGEEGLEEELDQCGIAHCGGSDPVDREFKAPIDFTVFKPDDSIGAVLCGFDSWINYQKLAKAMTYLRNPECKLILTNTDPTFPTHGDVFPGSGSLSIPIVNASKRKPLVIGKPNKMMMDAILAHHMFDPSRALMVGDNLATDIAFGRNSKIRTLLVMGGVTKYEQVFGENPNEVVPDLVMNSFGDLAMLADASEQ | |||||||||
Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 3753 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 5989 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 5895 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 7064 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 7111 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 2162 |
Gene Disruption Strategy
| Primer Name | Description | Primer Sequence |
|---|---|---|
| L1 | CNAG_03957 5' flanking region primer 1 | TCAACCAGTCTTCCAGTGG |
| L2 | CNAG_03957 5' flanking region primer 2 | TCACTGGCCGTCGTTTTACATTTGAGGAAAGGAGGGGC |
| R1 | CNAG_03957 3' flanking region primer 1 | CATGGTCATAGCTGTTTCCTGATGCGTCTGAGCAATAGGG |
| R2 | CNAG_03957 3' flanking region primer 2 | ACAGATGACGAGGTAAGGC |
| SO | CNAG_03957 diagnostic screening primer, pairing with B79 | TTTGAGGAAGCCGTAGAGACCC |
| PO | CNAG_03957 Southern blot probe primer | AGAAGCGTTCAAGCCAAGC |
| STM | NAT#5 STM primer | TGCTAGAGGGCGGGAGAGTT |
| STM common | STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
* Red marked sequences indicate the extended M13 forward and reversed sequences to overlap PCR with pNAT plasmid.